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Literature summary for 3.2.1.98 extracted from

  • Li, Z.; Wu, J.; Zhang, B.; Wang, F.; Ye, X.; Huang, Y.; Huang, Q.; Cui, Z.
    AmyM, a novel maltohexaose-forming alpha-amylase from Corallococcus sp. strain EGB (2015), Appl. Environ. Microbiol., 81, 1977-1987 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.98

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 30 mg/ml, 1.2fold activation Corallococcus sp. EGB

Application

Application Comment Organism
industry applications in starch processing technologies because of its highly specific activity, halotolerance, unique substrate specificity, and end product pattern Corallococcus sp. EGB

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) with a hexahistidine tag Corallococcus sp. EGB

General Stability

General Stability Organism
the enzyme is highly tolerant to high concentrations of salts, detergents, and various organic solvents Corallococcus sp. EGB
the enzyme is still active in the presence of high salt concentrations, with 74% and 88% of its activity being retained in 1 M NaCl and KCl solutions, respectively, and 67% of its activity being retained in a 3 M KCl solution. The enzymatic activity is reduced when the NaCl concentration is 2 M Corallococcus sp. EGB

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile 20%, 38.6% loss of activity Corallococcus sp. EGB
Ba2+ 1 mM, 11.1% loss of activity Corallococcus sp. EGB
Co2+ 1 mM, 68.3% loss of activity Corallococcus sp. EGB
Cr3+ 1 mM, 10.1% loss of activity Corallococcus sp. EGB
Cu+ 1 mM, 83.1% loss of activity Corallococcus sp. EGB
Cu2+ 1 mM, 89.8% loss of activity Corallococcus sp. EGB
DMSO 10%, 18.3% loss of activity Corallococcus sp. EGB
EDTA 10 mM, 83% loss of activity Corallococcus sp. EGB
ethanol 20%, 29.5% loss of activity Corallococcus sp. EGB
Fe2+ 1 mM, 74.7% loss of activity Corallococcus sp. EGB
Fe3+ 1 mM, 85.9% loss of activity Corallococcus sp. EGB
methanol 20%, 22.4% loss of activity Corallococcus sp. EGB
Mn2+ 1 mM, 35.2% loss of activity Corallococcus sp. EGB
Ni2+ 1 mM, 59.7% loss of activity Corallococcus sp. EGB
SDS 2 mg/ml, 46.5% loss of activity Corallococcus sp. EGB
Zn2+ 1 mM, 91.9% loss of activity Corallococcus sp. EGB

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 soluble starch Km-value: 6.61 mg/ml, pH 7.0, 50°C Corallococcus sp. EGB

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, activation to 109% of control Corallococcus sp. EGB
K+ 1 mM, activation to 127% of control Corallococcus sp. EGB
Mg2+ 1 mM, activation to 117% of control Corallococcus sp. EGB

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 SDS-PAGE Corallococcus sp. EGB
55460
-
 calculated from sequence Corallococcus sp. EGB

Organism

Organism UniProt Comment Textmining
Corallococcus sp. EGB
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetic acid affinity chromatography Corallococcus sp. EGB

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Corallococcus sp. EGB ?
-
 ?
amylose + H2O
-
 Corallococcus sp. EGB ?
-
 ?
corn starch + H2O
-
 Corallococcus sp. EGB ?
-
 ?
gamma-dextrin + H2O
-
 Corallococcus sp. EGB ?
-
 ?
glycogen + H2O
-
 Corallococcus sp. EGB ?
-
 ?
additional information no reaction is observed for pullulan, dextran, and alpha- or beta-cyclodextrins Corallococcus sp. EGB ?
-
 ?
soluble starch + H2O maltohexaose accounts for 59.4% of the maltooligosaccharides produced. Glucose (3.9%), maltose (6.2%), maltotriose (8.1%), maltotetraose (8.3%), maltopentaose (8.8%) and maltoheptaose (5.3%) Corallococcus sp. EGB maltohexaose + maltopentaose + maltose + D-glucose + maltotriose + maltotetraose
-
 ?
sweet potato starch + H2O
-
 Corallococcus sp. EGB ?
-
 ?
waxy maize starch + H2O
-
 Corallococcus sp. EGB maltohexaose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Corallococcus sp. EGB

Synonyms

Synonyms Comment Organism
AmyM
-
 Corallococcus sp. EGB

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 optimally active at 50°C in Tris-HCl buffer (50 mM, pH 7.0) Corallococcus sp. EGB

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 50% of maximal activity, 70°C: about 30% of maximal activity Corallococcus sp. EGB

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable below Corallococcus sp. EGB

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Corallococcus sp. EGB

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 30% of maximal activity, pH 9.0: about 40% of maximal activity Corallococcus sp. EGB

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable Corallococcus sp. EGB

pI Value

Organism Comment pI Value Maximum pI Value
Corallococcus sp. EGB calculated from sequence
-
 6.45