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Literature summary for 3.2.1.98 extracted from

  • Kanai, R.; Haga, K.; Akiba, T.; Yamane, K.; Harata, K.
    Biochemical and crystallographic analyses of maltohexaose-producing amylase from alkalophilic Bacillus sp. 707 (2004), Biochemistry, 43, 14047-14056.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus subtilis strain 207-25 Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) P19571 alkalophilic strain 707
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
starch, culture medium, pH 7.6 Bacillus sp. (in: Bacteria)
1.5
-
starch, purified enzyme, pH 7.6 Bacillus sp. (in: Bacteria)
18.8
-
starch, purified enzyme, pH 8.8 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose + H2O
-
Bacillus sp. (in: Bacteria) maltohexaose + maltotriose + maltoheptaose
-
?
maltoheptaose + H2O
-
Bacillus sp. (in: Bacteria) maltohexaose + maltotriose + maltotetraose + maltopentaose + maltose + D-glucose
-
?
starch + H2O
-
Bacillus sp. (in: Bacteria) maltohexaose + ?
-
?

Synonyms

Synonyms Comment Organism
G6-amylase
-
Bacillus sp. (in: Bacteria)
maltohexaose-producing alpha-amylase
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 70 activity decreases to less than 20% above 55°C Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
activity decreases to less than 20% above 55°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4.7 10.8
-
Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.7 10.8 retaining more than 50% of starch-degrading activity at 37°C Bacillus sp. (in: Bacteria)