Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis strain 207-25 | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | P19571 | alkalophilic strain 707 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
starch, culture medium, pH 7.6 | Bacillus sp. (in: Bacteria) |
1.5 | - |
starch, purified enzyme, pH 7.6 | Bacillus sp. (in: Bacteria) |
18.8 | - |
starch, purified enzyme, pH 8.8 | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + H2O | - |
Bacillus sp. (in: Bacteria) | maltohexaose + maltotriose + maltoheptaose | - |
? | |
maltoheptaose + H2O | - |
Bacillus sp. (in: Bacteria) | maltohexaose + maltotriose + maltotetraose + maltopentaose + maltose + D-glucose | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) | maltohexaose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6-amylase | - |
Bacillus sp. (in: Bacteria) |
maltohexaose-producing alpha-amylase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | activity decreases to less than 20% above 55°C | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
activity decreases to less than 20% above 55°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | 10.8 | - |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.7 | 10.8 | retaining more than 50% of starch-degrading activity at 37°C | Bacillus sp. (in: Bacteria) |