BRENDA - Enzyme Database show
show all sequences of 3.2.1.98

Enzymatic synthesis of p-nitrophenyl alpha-maltoheptaoside by transglycosylation of maltohexaose-forming amylase

Ogawa, K.; Uejima, O.; Nakakuki, T.; Usui, T.; Kainuma, K.; Agric. Biol. Chem. 54, 581-586 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ethanol
transglycosylation, activation
Klebsiella pneumoniae
methanol
aqueous methanol system, up to 40% methanol, increases transglycosylation
Klebsiella pneumoniae
Application
Application
Commentary
Organism
synthesis
enzymatic reaction, transglycosylation, provides practical technique for industrial production of p-nitrophenyl alpha-maltoheptaoside, useful substrate for assay of human alpha-amylase in serum and urine
Klebsiella pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-propanol
transglycosylation, inactivation
Klebsiella pneumoniae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
ultraviolet induced mutant
Klebsiella pneumoniae
-
-
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Ethanol
stable and activation
Klebsiella pneumoniae
Methanol
stable up to 40%, at 50% methanol: inactivation of transglycosylation and hydrolysis
Klebsiella pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
Aerobacter aerogenes; ultraviolet-induced mutant
-
Reaction
Reaction
Commentary
Organism
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltohexaose residues from the non-reducing chain ends
mechanism of transglycosylation
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amylose + H2O
short-chain amylose, DP, average degree of polymerization, = 23
171731
Klebsiella pneumoniae
maltohexaose
main product, 40% maltohexaose
171731
Klebsiella pneumoniae
?
maltoheptaose + p-nitrophenyl alpha-glucoside
transglycosylation, mechanism
171731
Klebsiella pneumoniae
p-nitrophenyl alpha-maltoheptaoside + D-glucose
p-nitrophenyl alpha-maltoheptaoside useful substrate for assay of human alpha-amylase in serum and urine
171731
Klebsiella pneumoniae
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
transglycosylation
Klebsiella pneumoniae
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
45
-
transglycosylation, below, considerable inactivation at
Klebsiella pneumoniae
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
transglycosylation, considerabe inactivation
Klebsiella pneumoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
transglycosylation
Klebsiella pneumoniae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
transglycosylation
Klebsiella pneumoniae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
transglycosylation, complete inactivation
Klebsiella pneumoniae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ethanol
transglycosylation, activation
Klebsiella pneumoniae
methanol
aqueous methanol system, up to 40% methanol, increases transglycosylation
Klebsiella pneumoniae
Application (protein specific)
Application
Commentary
Organism
synthesis
enzymatic reaction, transglycosylation, provides practical technique for industrial production of p-nitrophenyl alpha-maltoheptaoside, useful substrate for assay of human alpha-amylase in serum and urine
Klebsiella pneumoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-propanol
transglycosylation, inactivation
Klebsiella pneumoniae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
ultraviolet induced mutant
Klebsiella pneumoniae
-
-
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Ethanol
stable and activation
Klebsiella pneumoniae
Methanol
stable up to 40%, at 50% methanol: inactivation of transglycosylation and hydrolysis
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amylose + H2O
short-chain amylose, DP, average degree of polymerization, = 23
171731
Klebsiella pneumoniae
maltohexaose
main product, 40% maltohexaose
171731
Klebsiella pneumoniae
?
maltoheptaose + p-nitrophenyl alpha-glucoside
transglycosylation, mechanism
171731
Klebsiella pneumoniae
p-nitrophenyl alpha-maltoheptaoside + D-glucose
p-nitrophenyl alpha-maltoheptaoside useful substrate for assay of human alpha-amylase in serum and urine
171731
Klebsiella pneumoniae
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
transglycosylation
Klebsiella pneumoniae
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
45
-
transglycosylation, below, considerable inactivation at
Klebsiella pneumoniae
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
transglycosylation, considerabe inactivation
Klebsiella pneumoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
transglycosylation
Klebsiella pneumoniae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
transglycosylation
Klebsiella pneumoniae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
transglycosylation, complete inactivation
Klebsiella pneumoniae
Other publictions for EC 3.2.1.98
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752207
Valk
Characterization of the starc ...
Microbacterium aurum, Microbacterium aurum B8.A
Sci. Rep.
6
36100
2016
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1
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11
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11
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749651
Li
AmyM, a novel maltohexaose-fo ...
Corallococcus sp. EGB
Appl. Environ. Microbiol.
81
1977-1987
2015
1
1
1
-
-
2
16
1
-
3
2
-
-
1
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1
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9
1
1
1
1
-
1
1
1
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1
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1
1
1
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2
-
16
-
1
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3
2
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1
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-
-
-
9
1
1
1
1
-
1
1
1
1
-
-
-
-
-
-
731818
Tran
Experimental evidence for a 9- ...
Bacillus licheniformis, Bacillus licheniformis ATCC 27811
FEBS Lett.
588
620-624
2014
-
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1
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1
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2
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1
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1
1
731665
Wang
-
Gene cloning, expression and e ...
Saccharomonospora viridis
Chin. J. Appl. Environ. Biol.
19
342-345
2013
-
1
-
-
-
-
-
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6
-
1
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6
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1
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1
1
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1
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6
-
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6
-
1
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1
1
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-
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-
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731903
Kumar
-
Studies on a maltohexaose (G6) ...
Streptomyces sp.
IIOAB J.
3
15-30
2012
2
1
-
-
-
-
1
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1
2
1
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1
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1
1
1
1
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1
1
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1
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2
1
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1
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1
2
1
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-
1
1
1
1
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-
1
1
-
1
1
-
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1
-
-
691421
Khemakhem
Engineering of the alpha-amyla ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus US100
Biotechnol. Bioeng.
102
380-389
2009
-
1
-
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1
-
-
-
-
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8
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1
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1
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2
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1
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1
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2
-
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1
-
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-
-
-
663976
Ben Ali
Thermostability enhancement an ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus US100
Biochem. J.
394
51-56
2006
-
-
1
-
-
-
-
-
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7
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1
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2
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1
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1
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2
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1
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1
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1
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-
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666865
Kanai
Role of Trp140 at subsite -6 o ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 707
Protein Sci.
15
468-477
2006
-
1
1
1
-
-
-
-
-
-
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3
-
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1
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1
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1
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1
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1
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1
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663490
Momma
Expression, crystallization an ...
Klebsiella pneumoniae
Acta Crystallogr. Sect. D
60
2352-2354
2004
-
-
1
1
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1
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3
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1
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664017
Kanai
Biochemical and crystallograph ...
Bacillus sp. (in: Bacteria)
Biochemistry
43
14047-14056
2004
-
-
1
1
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-
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3
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1
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3
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3
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1
1
1
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1
1
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1
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1
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1
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3
-
3
-
1
1
1
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1
1
1
-
-
-
-
-
-
-
664776
Ben Messaoud
-
Purification and properties of ...
Bacillus subtilis, Bacillus subtilis US116
Enzyme Microb. Technol.
34
662-666
2004
-
1
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1
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2
-
1
-
1
-
1
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-
-
-
-
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650619
Momma
Cloning and sequencing of the ...
Klebsiella pneumoniae
Biosci. Biotechnol. Biochem.
64
428-431
2000
-
-
1
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-
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1
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3
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1
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1
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171731
Ogawa
Enzymatic synthesis of p-nitro ...
Klebsiella pneumoniae
Agric. Biol. Chem.
54
581-586
1990
2
1
-
-
-
-
1
-
1
-
-
-
2
3
-
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1
-
-
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2
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1
1
1
-
1
1
1
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2
1
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1
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-
1
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-
2
-
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-
-
-
2
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
171740
Shirokizawa
Nucleotide sequence of the G6- ...
Bacillus sp. H-167
FEMS Microbiol. Lett.
70
131-135
1990
-
-
1
-
-
-
-
-
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3
-
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2
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1
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1
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1
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1
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3
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1
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1
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171736
Hayashi
-
Production and purification of ...
Bacillus sp. H-167
Agric. Biol. Chem.
52
443-448
1988
-
1
-
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-
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3
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1
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1
1
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1
3
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10
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1
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3
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1
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1
3
-
10
-
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-
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-
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-
171732
Nakakuki
-
Action patterns of various exo ...
Klebsiella pneumoniae
Carbohydr. Res.
128
297-310
1984
-
-
-
-
-
-
-
-
1
-
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1
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1
1
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10
-
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1
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1
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10
-
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-
171733
Monma
-
Formation and hydrolysis of ma ...
Klebsiella pneumoniae
Agric. Biol. Chem.
47
1769-1774
1983
-
-
-
-
-
-
-
5
1
-
-
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1
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1
1
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9
-
-
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-
-
-
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-
-
-
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-
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5
1
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1
-
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-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
171735
Nakakuki
Immobilization of the exo-malt ...
Klebsiella pneumoniae
Biotechnol. Bioeng.
25
1095-1107
1983
-
1
-
-
-
2
15
1
1
7
-
-
-
1
-
-
1
-
-
-
1
-
6
-
2
1
3
-
1
2
4
-
-
-
-
-
1
-
-
-
-
2
-
15
-
1
1
7
-
-
-
-
-
1
-
-
1
-
6
-
2
1
3
-
1
2
4
-
-
-
-
-
-
-
171738
Nakakuki
-
Purification and some properti ...
Klebsiella pneumoniae, Klebsiella pneumoniae IFO-3321
J. Jpn. Soc. Starch Sci.
29
188-197
1982
-
-
-
-
-
1
11
-
2
6
3
-
-
3
-
-
1
1
-
1
2
-
18
1
2
-
3
-
2
2
5
-
-
-
-
-
-
-
-
-
-
1
-
11
-
-
2
6
3
-
-
-
-
1
-
1
2
-
18
1
2
-
3
-
2
2
5
-
-
-
-
-
-
-
171739
Nakakuki
-
exo-Maltohexaohydrolase. Recen ...
Klebsiella pneumoniae, Klebsiella pneumoniae IFO-3321
J. Jpn. Soc. Starch Sci.
29
138-144
1982
-
-
-
-
-
1
-
-
2
-
3
-
-
3
-
-
1
1
-
1
1
-
4
-
2
-
2
-
2
1
3
-
-
-
-
-
-
-
-
-
-
1
-
-
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2
-
3
-
-
-
-
1
-
1
1
-
4
-
2
-
2
-
2
1
3
-
-
-
-
-
-
-
171741
Takasaki
-
Production of maltohexaose by ...
Bacillus circulans, Bacillus circulans G-6
Agric. Biol. Chem.
46
1539-1547
1982
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-
-
-
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16
1
1
1
1
-
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2
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1
1
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1
1
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11
-
1
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1
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1
1
1
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16
-
1
1
1
1
-
-
-
-
1
-
1
1
-
11
-
1
-
1
-
1
1
1
-
-
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-
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171742
Taniguchi
-
Characterization of a potato s ...
Bacillus circulans, Bacillus circulans F-2
Agric. Biol. Chem.
46
2107-2115
1982
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-
-
-
-
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1
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3
-
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1
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10
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-
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1
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1
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10
-
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171734
Kainuma
Purification and some properti ...
Klebsiella pneumoniae
Biochim. Biophys. Acta
410
333-346
1975
1
-
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-
-
2
11
-
1
2
1
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3
-
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1
1
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1
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14
-
1
1
4
-
1
2
3
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1
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-
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2
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11
-
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1
2
1
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1
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1
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14
-
1
1
4
-
1
2
3
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171737
Kainuma
Isolation and action pattern o ...
Enterobacter cloacae, Klebsiella pneumoniae
FEBS Lett.
26
281-285
1972
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-
-
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1
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3
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1
1
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11
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1
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2
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1
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1
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1
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11
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1
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2
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1
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