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Literature summary for 3.2.1.97 extracted from

  • Goda, H.M.; Ushigusa, K.; Ito, H.; Okino, N.; Narimatsu, H.; Ito, M.
    Molecular cloning, expression, and characterization of a novel endo-alpha-N-acetylgalactosaminidase from Enterococcus faecalis (2008), Biochem. Biophys. Res. Commun., 375, 541-546.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET23a and expressed with a histidine-tag in Escherichia coli BL21(DE3) Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM greatly inhibits Enterococcus faecalis
additional information 1 mM of Mg2+, Ni2+, Ca2+, Co2+, Na+, Mn2+, Fe2+, Fe3+, EGTA, and EDTA have little effect on the enzyme activity Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0596
-
Galbeta1-3GalNAcalpha-pNP
-
Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
1 * 150000, SDS-PAGE Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q833X5
-
-
Enterococcus faecalis NBRC3971 Q833X5
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
-
Enterococcus faecalis 4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
-
Enterococcus faecalis NBRC3971 4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
?
Galbeta1-3GalNAcalpha-pNP + H2O
-
Enterococcus faecalis Galbeta1-3GalNAc + p-nitrophenol
-
?
Galbeta1-3GalNAcalpha-pNP + H2O
-
Enterococcus faecalis NBRC3971 Galbeta1-3GalNAc + p-nitrophenol
-
?
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
-
Enterococcus faecalis ?
-
?
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
-
Enterococcus faecalis NBRC3971 ?
-
?
Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O
-
Enterococcus faecalis ?
-
?
Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O
-
Enterococcus faecalis NBRC3971 ?
-
?
Glc-NAcbeta1-3GalNAcalpha1-pNP + H2O
-
Enterococcus faecalis ?
-
?
additional information can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP Enterococcus faecalis ?
-
?
additional information can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP Enterococcus faecalis NBRC3971 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 150000, SDS-PAGE Enterococcus faecalis

Synonyms

Synonyms Comment Organism
endo-alpha-N-acetylgalactosaminidase
-
Enterococcus faecalis
endo-EF
-
Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Enterococcus faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49.9
-
Galbeta1-3GalNAcalpha-pNP
-
Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Enterococcus faecalis