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Literature summary for 3.2.1.97 extracted from

  • Yoshimoto, T.; Takahashi, E.; Tsuru, D.
    Purification and enzymatic properties of an endo-alpha-N-acetylgalactosaminidase from Bacillus laterosporus SS-16 (1990), J. Ferment. Bioeng., 70, 348-350.
No PubMed abstract available
Search for more literature for 3.2.1.97

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 5 mM, 35% inhibition Brevibacillus laterosporus
C2H5HgCl 5 mM, 23% inhibition Brevibacillus laterosporus
Ca2+ 5 mM, 26% inhibition Brevibacillus laterosporus
Cd2+ 5 mM, 22% inhibition Brevibacillus laterosporus
CH3HgCl 5 mM, 18% inhibition Brevibacillus laterosporus
Co2+ 5 mM, 18% inhibition Brevibacillus laterosporus
Cr3+ 5 mM, 58% inhibition Brevibacillus laterosporus
Cu2+ 5 mM, 20% inhibition Brevibacillus laterosporus
DFP 1 mM, 48% inhibition Brevibacillus laterosporus
Fe3+ 5 mM, 15% inhibition Brevibacillus laterosporus
galactonolactone 5 mM, 11% inhibition Brevibacillus laterosporus
glucose 5 mM, 11% inhibition Brevibacillus laterosporus
Hg2+ 5 mM, 20% inhibition Brevibacillus laterosporus
iodoacetate 5 mM, 17% inhibition Brevibacillus laterosporus
Mg2+ 5 mM, 17% inhibition Brevibacillus laterosporus
Mn2+ 5 mM, 22% inhibition Brevibacillus laterosporus
N-bromosuccinimide 1 mM, complete inhibition Brevibacillus laterosporus
PCMB 1 mM, 26% inhibition Brevibacillus laterosporus
Zn2+ 5 mM, 9% inhibition Brevibacillus laterosporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 asialofetuin
-
 Brevibacillus laterosporus
0.78
-
 asialo-kappa-casein
-
 Brevibacillus laterosporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 4 * 48000, SDS-PAGE Brevibacillus laterosporus
210000
-
 gel filtration Brevibacillus laterosporus

Organism

Organism UniProt Comment Textmining
Brevibacillus laterosporus
-
-
-
Brevibacillus laterosporus SS-16
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brevibacillus laterosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asialo kappa-casein + H2O
-
 Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
asialo kappa-casein + H2O
-
 Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?
asialo-kappa-casein + H2O
-
 Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
asialo-kappa-casein + H2O
-
 Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?
asialofetuin + H2O
-
 Brevibacillus laterosporus additional information
-
 ?
asialofetuin + H2O
-
 Brevibacillus laterosporus SS-16 additional information
-
 ?
asialoglycophorin + H2O
-
 Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
asialoglycophorin + H2O
-
 Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?
glycoprotein + H2O hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins Brevibacillus laterosporus Galbeta(1-3)GalNAc + ?
-
 ?
glycoprotein + H2O hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins Brevibacillus laterosporus SS-16 Galbeta(1-3)GalNAc + ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Brevibacillus laterosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 37
-
 Brevibacillus laterosporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 1 h, stable below Brevibacillus laterosporus
70
-
 1 h, complete inactivation Brevibacillus laterosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Brevibacillus laterosporus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 9 37°C, 1 h, stable Brevibacillus laterosporus