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Literature summary for 3.2.1.96 extracted from
- Expression, purification, and characterization of endo-beta-N-acetylglucosaminidase H using baculovirus-mediated silkworm protein expression system (2014), Appl. Biochem. Biotechnol., 172, 3978-3988.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in a silkworm-baculovirus expression system | Streptomyces plicatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32900 | - |
x * 32900, calculated from amino acid sequence | Streptomyces plicatus |
| 33000 | - |
x * 33000, SDS-PAGE | Streptomyces plicatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces plicatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography and StrepTrap column chromatography | Streptomyces plicatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme hydrolyzes GlcNAcbeta1->4GlcNAc linkage of high mannose and hybrid type glycans and cannot cleave the linkage of complex type glycans | Streptomyces plicatus | ? | - |
? |  |
| N-glycosylated RNase B + H2O | the enzyme cleaves within the chitobiose core of high-mannose oligosaccharides from RNase B | Streptomyces plicatus | ? | - |
? | |
| N-glycosylated silkworm hemolymph glycoproteins + H2O | - |
Streptomyces plicatus | ? | - |
? | |
| N-glycosylated storage protein 2 + H2O | - |
Streptomyces plicatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Streptomyces plicatus |
| ? | x * 32900, calculated from amino acid sequence | Streptomyces plicatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Endo H | - |
Streptomyces plicatus |
| endo-beta-N-acetylglucosaminidase H | - |
Streptomyces plicatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 47 | the enzymatic activity of the recombinant enzyme is retained at 27-47°C | Streptomyces plicatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | the enzymatic activity of the recombinant enzyme is retained at pH 4.0-7.0 | Streptomyces plicatus |
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Release 2023.1 (January 2023)
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