Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
(Man)6(GlcNAc)2-pyridylamidated | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gallus gallus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
1 * 52000, SDS-PAGE in presence of 2-mercaptoethanol | Gallus gallus |
54000 | - |
gel filtration | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gallus gallus | in cytosol the enzyme contributes to the production of free oligosaccharides with one reducing end N-acetylglucosamine residue in cooperation with neutral alpha-mannosidase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
hen | - |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oviduct | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
4°C, loss of activity within two to three days | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Man)6(GlcNAc)2-pyridylamidated + H2O | - |
Gallus gallus | ? | - |
? | |
additional information | specificity | Gallus gallus | ? | - |
? | |
additional information | the enzyme hydrolyzes oligomannose-type sugar chains faster than complex-type chains and hybrid-type chains. Sugar chains containing the manalpha1-2Manalpha1-3Manbeta1-4GlcNAcbeta1-GlcNAc structure are good substrates | Gallus gallus | ? | - |
? | |
additional information | in cytosol the enzyme contributes to the production of free oligosaccharides with one reducing end N-acetylglucosamine residue in cooperation with neutral alpha-mannosidase | Gallus gallus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE in presence of 2-mercaptoethanol | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
Endo-HO | - |
Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | pH 4.5: about 25% of maximal activity, pH 8.5: about 30% of maximal activity | Gallus gallus |