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Literature summary for 3.2.1.96 extracted from

  • Fujisaki, M.; Tsuchida, T.; Kadowaki, S.; Yamamoto, K.; Tochikura, T.
    Purification and properties of Acinetobacter sp. Endo-beta-N-acetylglucosaminidase acting on complex oligosaccharides of glycoproteins (1991), J. Ferment. Bioeng., 71, 242-247.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM HgCl2, about 50% inhibition Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
dansyl asialotransferrin glycopeptide
-
Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl asialotransferrin glycopeptide + H2O
-
Acinetobacter sp. ?
-
?
glycopeptide + H2O high activity on dansyl transferrin glycopeptide, can also hydrolyze dansyl asialotransferrin glycopeptide and dansyl transferrin glycopeptide containing complex type sugar chains Acinetobacter sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, 10 mM phosphate buffer, pH 7.0, stable below Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 3.5
-
Acinetobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 about 30% of maxiaml activity at pH 2.5 and at pH 5.0 Acinetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 4°C, 5 d, stable Acinetobacter sp.