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Literature summary for 3.2.1.96 extracted from

  • Tarentino, A.L.; Maley, F.
    Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus (1974), J. Biol. Chem., 249, 811-817.
    View publication on PubMed
Search for more literature for 3.2.1.96

General Stability

General Stability Organism
pronase, trypsin, and chymotrypsin at 2.5 mg per ml, when incubated with 0.005-0.010 mg of enzyme per ml, has little or no effect after 5 h at 37°C. 5 mg/ml pronase inactivates slowly during a 12 h period Streptomyces griseus
stable to repeated freezing and thawing or lyophilization Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM HgCl2, 60% loss of activity Streptomyces griseus
PCMB 1 mM, 30% loss of activity Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 dansyl-Asn-(GlcNAc)4(Man)6
-
 Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
 gel filtration Streptomyces griseus
27200
-
 equilibrium sedimentation Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification contains no carbohydrate Streptomyces griseus

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces griseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
 Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAc)2(Man)6(GlcNAc)2 + H2O
-
 Streptomyces griseus GlcNAc + (GlcNAc)1(Man)6(GlcNAc)2
-
 ?
Asn(GlcNAc)2-(Man)5 + H2O
-
 Streptomyces griseus AsnGlcNAc + Man5(GlcNAc)
-
 ?
Asn(GlcNAc)2-(Man)6 + H2O
-
 Streptomyces griseus AsnGlcNAc + (GlcNAc)1-(Man)6
-
 ?
Asn(GlcNAc)2-(Man)6(GlcNAc)2 + H2O
-
 Streptomyces griseus AsnGlcNAc + (GlcNAC)1-(Man)6(GlcNAc)2
-
 ?
Asn(GlcNAc)4(Man)6 + H2O
-
 Streptomyces griseus ?
-
 ?
dansyl-Asn-(GlcNAc)2(Man)6(GlcNAc)2 + H2O
-
 Streptomyces griseus ?
-
 ?
dansyl-Asn-(GlcNAc)4(Man)6 + H2O
-
 Streptomyces griseus dansyl-Asn-GlcNAc + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 48 h, stable Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 hydrolysis of dansyl-Asn-(GlcNAc)4(Man)6 Streptomyces griseus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 37°C, rapid inactivation below Streptomyces griseus
4.5 8.5 37°C, stable between pH 4.5 and pH 8.5, rapid loss of activity below pH 4.5 Streptomyces griseus