General Stability | Organism |
---|---|
pronase, trypsin, and chymotrypsin at 2.5 mg per ml, when incubated with 0.005-0.010 mg of enzyme per ml, has little or no effect after 5 h at 37°C. 5 mg/ml pronase inactivates slowly during a 12 h period | Streptomyces griseus |
stable to repeated freezing and thawing or lyophilization | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM HgCl2, 60% loss of activity | Streptomyces griseus | |
PCMB | 1 mM, 30% loss of activity | Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
dansyl-Asn-(GlcNAc)4(Man)6 | - |
Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23500 | - |
gel filtration | Streptomyces griseus |
27200 | - |
equilibrium sedimentation | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | contains no carbohydrate | Streptomyces griseus |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(GlcNAc)2(Man)6(GlcNAc)2 + H2O | - |
Streptomyces griseus | GlcNAc + (GlcNAc)1(Man)6(GlcNAc)2 | - |
? | |
Asn(GlcNAc)2-(Man)5 + H2O | - |
Streptomyces griseus | AsnGlcNAc + Man5(GlcNAc) | - |
? | |
Asn(GlcNAc)2-(Man)6 + H2O | - |
Streptomyces griseus | AsnGlcNAc + (GlcNAc)1-(Man)6 | - |
? | |
Asn(GlcNAc)2-(Man)6(GlcNAc)2 + H2O | - |
Streptomyces griseus | AsnGlcNAc + (GlcNAC)1-(Man)6(GlcNAc)2 | - |
? | |
Asn(GlcNAc)4(Man)6 + H2O | - |
Streptomyces griseus | ? | - |
? | |
dansyl-Asn-(GlcNAc)2(Man)6(GlcNAc)2 + H2O | - |
Streptomyces griseus | ? | - |
? | |
dansyl-Asn-(GlcNAc)4(Man)6 + H2O | - |
Streptomyces griseus | dansyl-Asn-GlcNAc + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
48 h, stable | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | hydrolysis of dansyl-Asn-(GlcNAc)4(Man)6 | Streptomyces griseus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
37°C, rapid inactivation below | Streptomyces griseus |
4.5 | 8.5 | 37°C, stable between pH 4.5 and pH 8.5, rapid loss of activity below pH 4.5 | Streptomyces griseus |