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Literature summary for 3.2.1.96 extracted from

  • Kimura, Y.; Tokuda, T.; Ohno, A.; Tanaka, H.; Ishiguro, Y.
    Enzymatic properties of endo-beta-N-acetylglucosaminidase from developing tomato fruits and soybean seeds: substrate specificity of plant origin endoglycosidase (1998), Biochim. Biophys. Acta, 1381, 27-36.
    View publication on PubMed
Search for more literature for 3.2.1.96

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 6 mM, 80% inhibition Glycine max
Zn2+ 6 mM, complete inhibition Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 gel filtration Glycine max
62000
-
 gel filtration Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max
-
 Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Solanum lycopersicum
-
seed
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Glycine max
additional information
-
-
 Solanum lycopersicum

Storage Stability

Storage Stability Organism
0°C, stable for at least 1 month Glycine max
0°C, stable for at least 1 month Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Man)3(Xyl)1(GlcNAc)2-pyridylamino + H2O
-
 Glycine max ?
-
 ?
(Man)3(Xyl)1(GlcNAc)2-pyridylamino + H2O
-
 Solanum lycopersicum ?
-
 ?
(Man)5(GlcNAc)2-pyridylamino + H2O
-
 Glycine max (Man)5(GlcNAc) + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
(Man)5(GlcNAc)2-pyridylamino + H2O
-
 Solanum lycopersicum (Man)5(GlcNAc) + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
(Man)6(GlcNAc)2-pyridylamino + H2O
-
 Glycine max ?
-
 ?
(Man)6(GlcNAc)2-pyridylamino + H2O
-
 Solanum lycopersicum ?
-
 ?
(Man)7(GlcNAc)2-pyridylamino + H2O
-
 Glycine max ?
-
 ?
(Man)7(GlcNAc)2-pyridylamino + H2O
-
 Solanum lycopersicum ?
-
 ?
(Man)8(GlcNAc)2-pyridylamino + H2O
-
 Glycine max ?
-
 ?
(Man)8(GlcNAc)2-pyridylamino + H2O
-
 Solanum lycopersicum ?
-
 ?
(Man)9(GlcNAc)2-pyridylamino + H2O
-
 Glycine max ?
-
 ?
(Man)9(GlcNAc)2-pyridylamino + H2O
-
 Solanum lycopersicum ?
-
 ?
beta-D-GlcNAc-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1,6)]-beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-pyridylamino
-
 Glycine max ?
-
 ?
beta-D-GlcNAc-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1,6)]-beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-pyridylamino
-
 Solanum lycopersicum ?
-
 ?
additional information specificity Glycine max ?
-
 ?
additional information specificity Solanum lycopersicum ?
-
 ?
additional information description of substrate specificity, enzyme barely hydrolyzes xylose-containing N-glycans (Man)3(Xyl)1(GlcNAc)2-pyridylamino and (Man)3(Fuc)1(Xyl)1(GlcNAc)2-pyridylamino Glycine max ?
-
 ?
additional information description of substrate specificity, enzyme barely hydrolyzes xylose-containing N-glycans (Man)3(Xyl)1(GlcNAc)2-pyridylamino and (Man)3(Fuc)1(Xyl)1(GlcNAc)2-pyridylamino Solanum lycopersicum ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-GM
-
 Glycine max
endo-LE
-
 Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 hydrolysis of (Man)6(GlcNAc)2-pyridylamino Glycine max
40
-
 hydrolysis of (Man)6(GlcNAc)2-pyridylamino Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 hydrolysis of (Man)6(GlcNAc)2-pyridylamino Solanum lycopersicum
6.5
-
-
 Solanum lycopersicum
6.5
-
 hydrolysis of (Man)6(GlcNAc)2-pyridylamino Glycine max