General Stability | Organism |
---|---|
retains nearly 30% of its activity in 1% SDS | Streptomyces plicatus |
stable to incubation with 250fold excess of either Pronase, chymotrypsin, or trypsin w/w. Slow inactivation by 1000fold excess of Pronase during 12 h incubation | Streptomyces plicatus |
stable to repeated freezing and thawing | Streptomyces plicatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
dansyl-Asn-(GlcNAc)4(Man)6 | - |
Streptomyces plicatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
equilibrium sedimentation analysis | Streptomyces plicatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces plicatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces plicatus |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 years | Streptomyces plicatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Asn(GlcNAc)2(Man)3 + H2O | - |
Streptomyces plicatus | ? | - |
? | |
Asn(GlcNAc)2-(Man)5 + H2O | - |
Streptomyces plicatus | AsnGlcNAc + Man5(GlcNAc) | - |
? | |
Asn(GlcNAc)2-(Man)6 + H2O | - |
Streptomyces plicatus | AsnGlcNAc + (GlcNAc)1-(Man)6 | - |
? | |
Asn(GlcNAc)2-(Man)6(GlcNAc)2 + H2O | - |
Streptomyces plicatus | AsnGlcNAc + (GlcNAC)1-(Man)6(GlcNAc)2 | - |
? | |
dansyl-Asn-(GlcNAc)4(Man)6 + H2O | - |
Streptomyces plicatus | dansyl-Asn-GlcNAc + ? | - |
? | |
additional information | no hydrolysis of dansyl-Asn(GlcNAc)2Man, the presence of fucose attached to the asparagine-proximal core glucosamine renders the di-N-acetylchitobiosyl moiety an inactive substrate. As the number of mannose residues is increased, the rate of hydrolysis increases to a maximum at five residues. Thereafter enzyme activity declines, but the rate is still substantial on mannosyl chains containing 54 residues | Streptomyces plicatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, SDS-PAGE | Streptomyces plicatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 4.5-8.5, protein concentration 0.0003 mg/ml, stable. Rapid loss of activity below pH 4.5 | Streptomyces plicatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | hydrolysis of dansyl-Asn-(GlcNAc)4(Man)6 | Streptomyces plicatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | 37°C, stable between pH 4.5 and pH 8.5, rapid loss of activity below pH 4.5 | Streptomyces plicatus |