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Literature summary for 3.2.1.96 extracted from

  • Tarentino, A.L.; Trimble, R.B.; Maley, F.
    endo-beta-N-Acetylglucosaminidase from Streptomyces plicatus (1978), Methods Enzymol., 50C, 574-580.
    View publication on PubMed

General Stability

General Stability Organism
retains nearly 30% of its activity in 1% SDS Streptomyces plicatus
stable to incubation with 250fold excess of either Pronase, chymotrypsin, or trypsin w/w. Slow inactivation by 1000fold excess of Pronase during 12 h incubation Streptomyces plicatus
stable to repeated freezing and thawing Streptomyces plicatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
dansyl-Asn-(GlcNAc)4(Man)6
-
Streptomyces plicatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
equilibrium sedimentation analysis Streptomyces plicatus

Organism

Organism UniProt Comment Textmining
Streptomyces plicatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces plicatus

Storage Stability

Storage Stability Organism
-20°C, stable for at least 2 years Streptomyces plicatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asn(GlcNAc)2(Man)3 + H2O
-
Streptomyces plicatus ?
-
?
Asn(GlcNAc)2-(Man)5 + H2O
-
Streptomyces plicatus AsnGlcNAc + Man5(GlcNAc)
-
?
Asn(GlcNAc)2-(Man)6 + H2O
-
Streptomyces plicatus AsnGlcNAc + (GlcNAc)1-(Man)6
-
?
Asn(GlcNAc)2-(Man)6(GlcNAc)2 + H2O
-
Streptomyces plicatus AsnGlcNAc + (GlcNAC)1-(Man)6(GlcNAc)2
-
?
dansyl-Asn-(GlcNAc)4(Man)6 + H2O
-
Streptomyces plicatus dansyl-Asn-GlcNAc + ?
-
?
additional information no hydrolysis of dansyl-Asn(GlcNAc)2Man, the presence of fucose attached to the asparagine-proximal core glucosamine renders the di-N-acetylchitobiosyl moiety an inactive substrate. As the number of mannose residues is increased, the rate of hydrolysis increases to a maximum at five residues. Thereafter enzyme activity declines, but the rate is still substantial on mannosyl chains containing 54 residues Streptomyces plicatus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Streptomyces plicatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 4.5-8.5, protein concentration 0.0003 mg/ml, stable. Rapid loss of activity below pH 4.5 Streptomyces plicatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 hydrolysis of dansyl-Asn-(GlcNAc)4(Man)6 Streptomyces plicatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 8.5 37°C, stable between pH 4.5 and pH 8.5, rapid loss of activity below pH 4.5 Streptomyces plicatus