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Literature summary for 3.2.1.96 extracted from

  • Yamamoto, K.; Kadowaki, S.; Takegawa, K.; Kumagai, H.; Tochikura, T.
    Purification and characterization of endo-beta-N-acetylglucosaminidase from a Flavobacterium sp. (1986), Agric. Biol. Chem., 50, 421-429.
No PubMed abstract available
Search for more literature for 3.2.1.96

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM HgCl2, 85% loss of activity Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 dansyl-Asn-(GlcNAc)2(Man)6
-
 Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Flavobacterium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Flavobacterium sp.
30000
-
 1 * 30000, SDS-PAGE Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.9
-
-
 Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-Asn-(GlcNAc)2(Man)6 + H2O
-
 Flavobacterium sp. dansyl-Asn-GlcNAc + (Man)6GlcNAc
-
 ?
ovalbumin + H2O native ovalbumin Flavobacterium sp. oligosaccharides + ?
-
 ?
ribonuclease B + H2O bovine pancreatic ribonuclease B Flavobacterium sp. ?
-
 ?
yeast invertase + H2O
-
 Flavobacterium sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, stable up to Flavobacterium sp.
80
-
 10 min, 65% loss of activity Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8 pH 2.5: about 20% of maximal activity, pH 8.0: about 30% of maximal activity Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 4°C, 2 days, stable Flavobacterium sp.