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Literature summary for 3.2.1.91 extracted from

  • Nakabayashi, M.; Kamachi, S.; Malle, D.; Yanamoto, T.; Kishishita, S.; Fujii, T.; Inoue, H.; Ishikawa, K.
    Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering (2019), Protein Eng. Des. Sel., 32, 33-40 .
    View publication on PubMed
Search for more literature for 3.2.1.91

Protein Variants

Protein Variants Comment Organism
A401P the mutant enzyme shows improved thermostability compared to the wild type enzyme Talaromyces pinophilus
A426P the mutant shows 86.9% of wild type activity Talaromyces pinophilus
S273P the mutant enzyme shows improved thermostability compared to the wild type enzyme Talaromyces pinophilus
S298P the mutant shows 88% of wild type activity Talaromyces pinophilus
T237G the mutant enzyme shows improved thermostability compared to the wild type enzyme Talaromyces pinophilus
T262G the mutant shows 94.4% of wild type activity Talaromyces pinophilus
T262G /S298P /A426P the mutant shows 95.7% of wild type activity Talaromyces pinophilus

Organism

Organism UniProt Comment Textmining
Talaromyces pinophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Talaromyces pinophilus 4-nitrophenol + beta-D-lactopyranose
-
 ?
avicel + H2O
-
 Talaromyces pinophilus ?
-
 ?
crystalline cellulose + H2O
-
 Talaromyces pinophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
CBHI
-
 Talaromyces pinophilus
cellobiohydrolase I
-
 Talaromyces pinophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Talaromyces pinophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
63
-
 the melting temperature is 63°C. The wild type enzyme is inactive after 30 min at 65°C Talaromyces pinophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Talaromyces pinophilus