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Literature summary for 3.2.1.91 extracted from
- celB, a gene coding for a bifunctional cellulase from the extreme thermophile "Caldocellum saccharolyticum" (1990), Appl. Environ. Microbiol., 56, 3117-3124.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caldicellulosiruptor saccharolyticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of deletion mutants expressing solely the aminoterminal domain containing the exoglucanase activity. Temperature optimum and stability of the deletion mutants are the same as wild-type | Caldicellulosiruptor saccharolyticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 118000 | 120000 | PAGE | Caldicellulosiruptor saccharolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | P10474 | bifunctional enzyme, shows both endoglucanase and exoglucanase activities, EC 3.2.1.4 and 3.2.1.91, respectively | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-methylumbelliferone + beta-D-cellobiose | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the exoglucanase activity is located in the amino-terminal domain of the enzyme and the endoglucanase activity is located in the carboxy-terminal domain | Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life 29 h | Caldicellulosiruptor saccharolyticus |
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Release 2023.1 (January 2023)
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