Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 | Humicola insolens |
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 | Trichoderma reesei |
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 | Thermochaetoides thermophila |
Protein Variants | Comment | Organism |
---|---|---|
A385P | the mutant shows a T50 value of 64.7°C | Humicola insolens |
M134L | the mutant shows a T50 value of 62°C | Trichoderma reesei |
M235P | the mutant shows a T50 value of 63.1°C | Humicola insolens |
Q276L | the mutant shows a T50 value of 58.6°C | Trichoderma reesei |
Q390P | the mutant shows a T50 value of 66.1°C | Humicola insolens |
S215P | the mutant shows a T50 value of 65.6°C | Humicola insolens |
S30F | the mutant shows a T50 value of 60.3°C | Trichoderma reesei |
S316P | the mutant shows a T50 value of 61.9°C | Trichoderma reesei |
S406P | the mutant shows a T50 value of 61.5°C | Trichoderma reesei |
S413P | the mutant shows a T50 value of 63.8°C | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | Humicola insolens | - |
cellobiose + ? | - |
? | |
cellulose + H2O | Trichoderma reesei | - |
cellobiose + ? | - |
? | |
cellulose + H2O | Thermochaetoides thermophila | - |
cellobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humicola insolens | - |
- |
- |
Thermochaetoides thermophila | - |
- |
- |
Trichoderma reesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Humicola insolens |
Ni-NTA column chromatography | Trichoderma reesei |
Ni-NTA column chromatography | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | - |
Humicola insolens | cellobiose + ? | - |
? | |
avicel + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? | |
avicel + H2O | - |
Thermochaetoides thermophila | cellobiose + ? | - |
? | |
cellulose + H2O | - |
Humicola insolens | cellobiose + ? | - |
? | |
cellulose + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? | |
cellulose + H2O | - |
Thermochaetoides thermophila | cellobiose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel6A | - |
Humicola insolens |
Cel6A | - |
Trichoderma reesei |
Cel6A | - |
Thermochaetoides thermophila |
cellobiohydrolase | - |
Humicola insolens |
cellobiohydrolase | - |
Trichoderma reesei |
cellobiohydrolase | - |
Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
the wild type enzyme is inactive at 75°C | Thermochaetoides thermophila |
75 | - |
the wild type enzyme shows a half-life of more than 2.5 min at 75°C and T50 of 60.2°C | Trichoderma reesei |
75 | - |
the wild type enzyme shows a half-life of more than 2.5 min at 75°C and T50 of 65.2°C | Humicola insolens |