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Literature summary for 3.2.1.91 extracted from

  • Brunecky, R.; Alahuhta, M.; Bomble, Y.J.; Xu, Q.; Baker, J.O.; Ding, S.Y.; Himmel, M.E.; Lunin, V.V.
    Structure and function of the Clostridium thermocellum cellobiohydrolase A X1-module repeat: enhancement through stabilization of the CbhA complex (2012), Acta Crystallogr. Sect. D, 68, 292-299.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using in 0.2 M ammonium iodide, 20% (w/v) polyethylene glycol 3350 pH 6.2 or 0.2 M CaCl2, 20% (w/v) polyethylene glycol 3350 pH 5.1 Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ contains Ca2+ Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Acetivibrio thermocellus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Source 15S column chromatography, and Superdex 75 gel filtration Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose + H2O
-
Acetivibrio thermocellus ?
-
?

Synonyms

Synonyms Comment Organism
CbhA
-
Acetivibrio thermocellus
cellobiohydrolase A
-
Acetivibrio thermocellus