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Literature summary for 3.2.1.89 extracted from

  • Sakamoto, T.; Nishimura, Y.; Makino, Y.; Sunagawa, Y.; Harada, N.
    Biochemical characterization of a GH53 endo-beta-1,4-galactanase and a GH35 exo-beta-1,4-galactanase from Penicillium chrysogenum (2013), Appl. Microbiol. Biotechnol., 97, 2895-2906.
    View publication on PubMed
Search for more literature for 3.2.1.89

Cloned(Commentary)

Cloned (Comment) Organism
gene Pcgal1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha Penicillium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Penicillium chrysogenum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE Penicillium chrysogenum
78000
-
 x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum B5MGR3 gene Pcgal1
-
Penicillium chrysogenum 31B B5MGR3 gene Pcgal1
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from the culture supernatant by ammonium sulfate fractionation, ultrafiltration, anion exchange and hydrophobic interaction chromatography, and gel filtration Penicillium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
 purified recombinant enzyme, substrate lupin galactan, pH 5.0, 37°C Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
debranched sugar beet arabinan + H2O low activity Penicillium chrysogenum ?
-
 ?
debranched sugar beet arabinan + H2O low activity Penicillium chrysogenum 31B ?
-
 ?
lupin beta-1,4-galactan + H2O best substrate Penicillium chrysogenum ?
-
 ?
lupin beta-1,4-galactan + H2O best substrate Penicillium chrysogenum 31B ?
-
 ?
additional information substrate specificity, overview. The smallest substrate for the enzyme is beta-1,4-galactotriose. No activity with sugar beet pectin, beta-1,3-galactan from larch wood, and soybean rhamnogalacturonan. No activity with 4-nitrophenly beta-D-galactopyranoside Penicillium chrysogenum ?
-
 ?
additional information substrate specificity, overview. The smallest substrate for the enzyme is beta-1,4-galactotriose. No activity with sugar beet pectin, beta-1,3-galactan from larch wood, and soybean rhamnogalacturonan. No activity with 4-nitrophenly beta-D-galactopyranoside Penicillium chrysogenum 31B ?
-
 ?
soybean arabinogalactan + H2O high activity Penicillium chrysogenum ?
-
 ?
soybean arabinogalactan + H2O high activity Penicillium chrysogenum 31B ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
endo-beta-1,4-galactanase
-
 Penicillium chrysogenum
PcGAL1
-
 Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant enzyme, pH 5.0, over 80% activity remaining after 1 h Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Penicillium chrysogenum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 purified recombinant enzyme, over 80% activity remaining after 16 h Penicillium chrysogenum

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 53, GH53 Penicillium chrysogenum
additional information the enzyme acts synergistically with the exo-beta-1,4-galactanase of Penicillium chrysogenum Penicillium chrysogenum