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Literature summary for 3.2.1.87 extracted from

  • Hsieh, P.F.; Lin, H.H.; Lin, T.L.; Chen, Y.Y.; Wang, J.T.
    Two T7-like bacteriophages, K5-2 and K5-4, each encodes two capsule depolymerases isolation and functional characterization (2017), Sci. Rep., 7, 4624 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Klebsiella phage K5-4
expressed in Escherichia coli BL21(DE3) cells Klebsiella phage K5-2

Organism

Organism UniProt Comment Textmining
Klebsiella phage K5-2 A0A219YHC0
-
-
Klebsiella phage K5-4 A0A219YHG0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Klebsiella phage K5-4
Ni-NTA column chromatography Klebsiella phage K5-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Klebsiella capsular polysaccharide + H2O
-
 Klebsiella phage K5-4 Klebsiella capsular monosaccharides
-
 ?
Klebsiella capsular polysaccharide + H2O
-
 Klebsiella phage K5-2 Klebsiella capsular monosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
capsule depolymerase
-
 Klebsiella phage K5-4
capsule depolymerase
-
 Klebsiella phage K5-2
K30/K69 depolymerase
-
 Klebsiella phage K5-4
K30/K69 depolymerase
-
 Klebsiella phage K5-2
K30/K69dep
-
 Klebsiella phage K5-4
K30/K69dep
-
 Klebsiella phage K5-2
K5 depolymerase
-
 Klebsiella phage K5-4
K5 depolymerase
-
 Klebsiella phage K5-2
K5dep
-
 Klebsiella phage K5-4
K5dep
-
 Klebsiella phage K5-2
K8 depolymerase
-
 Klebsiella phage K5-4
K8 depolymerase
-
 Klebsiella phage K5-2
K8dep
-
 Klebsiella phage K5-4
K8dep
-
 Klebsiella phage K5-2
tail fiber protein
-
 Klebsiella phage K5-4
tail fiber protein
-
 Klebsiella phage K5-2

General Information

General Information Comment Organism
physiological function the enzyme is able to infect and grow on either capsular types K30/K69 and K5 or K8 and K5 of Klebsiella strains. Bacterial killing is observed when incubated Klebsiella strains with phages but not with purified enzymes Klebsiella phage K5-4
physiological function the enzyme is able to infect and grow on either capsular types K30/K69 and K5 or K8 and K5 of Klebsiella strains. Bacterial killing is observed when incubated Klebsiella strains with phages but not with purified enzymes Klebsiella phage K5-2