Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.87 extracted from

  • Liu, Y.; Mi, Z.; Mi, L.; Huang, Y.; Li, P.; Liu, H.; Yuan, X.; Niu, W.; Jiang, N.; Bai, C.; Gao, Z.
    Identification and characterization of capsule depolymerase Dpo48 from Acinetobacter baumannii phage IME200 (2019), PeerJ, 7, e6173 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acinetobacter phage IME-200

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacterial surface polysaccharide + H2O Acinetobacter phage IME-200
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter phage IME-200 A0A0P0IJ73
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Acinetobacter phage IME-200

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacterial surface polysaccharide + H2O
-
 Acinetobacter phage IME-200 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Acinetobacter phage IME-200
? x * 75141, calculated from amino acid sequence Acinetobacter phage IME-200

Synonyms

Synonyms Comment Organism
capsule depolymerase
-
 Acinetobacter phage IME-200
Dpo48
-
 Acinetobacter phage IME-200
polysaccharide depolymerase
-
 Acinetobacter phage IME-200
tail fiber protein
-
 Acinetobacter phage IME-200

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Acinetobacter phage IME-200

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 the enzyme maintains high activity at temperatures ranging from 20 to 70°C. The enzymatic activity diminishes at 80°C Acinetobacter phage IME-200

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Acinetobacter phage IME-200

pH Range

pH Minimum pH Maximum Comment Organism
5 9 the enzyme maintains high activity at temperatures ranging from pH 5.0 to 9.0 Acinetobacter phage IME-200

General Information

General Information Comment Organism
physiological function the enzyme degrades the bacterial surface exopolysaccharides of Actinetobacter baumannii types 208, 238, 420 and 1106 Acinetobacter phage IME-200