Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.87 extracted from

  • Lin, H.; Paff, M.L.; Molineux, I.J.; Bull, J.J.
    Therapeutic application of phage capsule depolymerases against K1, K5, and K30 capsulated E. coli in mice (2017), Front. Microbiol., 8, 2257 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.87

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
K30 capsule polysaccharide + H2O Escherichia phage K30
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia phage K1
-
-
-
Escherichia phage K30
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisPur Ni-NTA resin column chromatography Escherichia phage K1
HisPur Ni-NTA resin column chromatography Escherichia phage K30

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
K1 capsule polysaccharide + H2O
-
 Escherichia phage K1 ?
-
 ?
K30 capsule polysaccharide + H2O
-
 Escherichia phage K30 ?
-
 ?

Subunits

Subunits Comment Organism
octadecamer 18 * 76000, isoform K1E, SDS-PAGE Escherichia phage K1
trimer 3 * 103000, isoform K1F, SDS-PAGE Escherichia phage K1
trimer 3 * 93000, isoform K1H, SDS-PAGE Escherichia phage K1
trimer 3 * 97000, SDS-PAGE Escherichia phage K30

Synonyms

Synonyms Comment Organism
K1E depolymerase isoform Escherichia phage K1
K1F depolymerase isoform Escherichia phage K1
K1H depolymerase isoform Escherichia phage K1
K30 depolymerase
-
 Escherichia phage K30
K30 gp41
-
 Escherichia phage K30
phage capsule depolymerase
-
 Escherichia phage K1
phage capsule depolymerase
-
 Escherichia phage K30

General Information

General Information Comment Organism
physiological function the enzyme actively degrades K1 capsule polysaccharide in vitro and sensitizes K1 bacteria to serum killing Escherichia phage K1