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Literature summary for 3.2.1.87 extracted from

  • Yurewicz, E.C.; Ghalambor, M.A.; Duckworth, D.H.; Heath, E.C.
    Catalytic and molecular properties of a phage-induced capsular polysaccharide depolymerase (1971), J. Biol. Chem., 246, 5607-5616.
    View publication on PubMed
Search for more literature for 3.2.1.87

Inhibitors

Inhibitors Comment Organism Structure
FeCl3
-
 Klebsiella aerogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Klebsiella aerogenes 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ion not required Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36400
-
 octamer: 4 * 63200 + 4 * 36400 or heptamer: 4 * 63200 + 3 * 36400, SDS-PAGE Klebsiella aerogenes
36400
-
 heptamer: 4 * 63200 + 3 * 36400 or octamer: 4 * 63200 + 4 * 36400, SDS-PAGE Klebsiella aerogenes
63200
-
 octamer: 4 * 63200 + 4 * 36400 or heptamer: 4 * 63200 + 3 * 36400, SDS-PAGE Klebsiella aerogenes
63200
-
 heptamer: 4 * 63200 + 3 * 36400 or octamer: 4 * 63200 + 4 * 36400, SDS-PAGE Klebsiella aerogenes
379000
-
 sedimentation coefficient, diffusion coefficient Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal]n + H2O Klebsiella aerogenes capsular, polysaccharide produced by Aerobacter aerogenes, hydrolyses the galactosyl-alpha-1,3-D-galactose linkages only in the complex substrate, bringing about depolymerization, the enzyme is phage-induced and serves to degrade the capsular polysaccharide of the host D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
 phage induced
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
235
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal]n + H2O capsular, polysaccharide produced by Aerobacter aerogenes, hydrolyzes the galactosyl-alpha-1,3-D-galactose linkages only in the complex substrate, bringing about depolymerization Klebsiella aerogenes D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal
-
 ?
[D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal]n + H2O capsular, polysaccharide produced by Aerobacter aerogenes, hydrolyses the galactosyl-alpha-1,3-D-galactose linkages only in the complex substrate, bringing about depolymerization, the enzyme is phage-induced and serves to degrade the capsular polysaccharide of the host Klebsiella aerogenes D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal
-
 ?

Subunits

Subunits Comment Organism
heptamer heptamer: 4 * 63200 + 3 * 36400 or octamer: 4 * 63200 + 4 * 36400, SDS-PAGE Klebsiella aerogenes
octamer octamer: 4 * 63200 + 4 * 36400 or heptamer: 4 * 63200 + 3 * 36400, SDS-PAGE Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Klebsiella aerogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
68
-
 52% inactivation after 10 min Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
3.2 8
-
 Klebsiella aerogenes