Literature summary for 3.2.1.86 extracted from

Terra, V.S.; Zhi, X.; Kahya, H.F.; Andrew, P.W.; Yesilkaya, H.
Pneumococcal 6-phospho-beta-glucosidase (BglA3) is involved in virulence and nutrient metabolism (2016), Infect. Immun., 84, 286-292 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.416	-	4-nitrophenyl 6-phospho-beta-glucoside	at pH 7.5 and 37°C	Streptococcus pneumoniae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Streptococcus pneumoniae	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-nitrophenyl 6-phospho-beta-glucoside + H2O	Streptococcus pneumoniae	-	4-nitrophenol + D-glucose 6-phosphate	-	?
additional information	Streptococcus pneumoniae	no activity with 2-nitrophenyl beta-D-galactopyranoside, 4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide, 4-methylumbelliferyl beta-D-glucopyranoside, 4-methylumbelliferyl alpha-D-glucopyranoside, 2-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucoside 6-phosphate, 4-nitrophenyl alpha-D-mannoside 6-phosphate, and 4-nitrophenyl alpha-D-galactoside 6-phosphate	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal affinity chromatography	Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.37	-	at pH 7.5 and 37°C	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl 6-phospho-beta-glucoside + H2O	-	Streptococcus pneumoniae	4-nitrophenol + D-glucose 6-phosphate	-	?
additional information	no activity with 2-nitrophenyl beta-D-galactopyranoside, 4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide, 4-methylumbelliferyl beta-D-glucopyranoside, 4-methylumbelliferyl alpha-D-glucopyranoside, 2-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucoside 6-phosphate, 4-nitrophenyl alpha-D-mannoside 6-phosphate, and 4-nitrophenyl alpha-D-galactoside 6-phosphate	Streptococcus pneumoniae	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 53200, calculated from amino acid sequence	Streptococcus pneumoniae
?	x * 53000-55000, SDS-PAGE	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
BglA3	-	Streptococcus pneumoniae
phospho-beta-glucosidase	-	Streptococcus pneumoniae
SPD_0247	-	Streptococcus pneumoniae

General Information

General Information	Comment	Organism
malfunction	an enzyme deletion mutant shows reduced 6-phospho-glucosidase activity and is attenuated in growth on cellobiose and hyaluronic acid compared to the growth of wild type. Mutant-infected mice survive significantly longer than the wild-type-infected cohort, and the colony counts of the mutant are lower than those of the wild type in the lungs. The enzyme deletion mutant is also severely impaired in attachment to an abiotic surface	Streptococcus pneumoniae

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Release 2023.1 (January 2023)