Literature summary for 3.2.1.86 extracted from

An, C.L.; Lim, W.J.; Hong, S.Y.; Shin, E.C.; Kim, M.K.; Lee, J.R.; Park, S.R.; Woo, J.G.; Lim, Y.P.; Yun, H.D.
Structural and biochemical analysis of the asc operon encoding 6-phospho-beta-glucosidase in Pectobacterium carotovorum subsp. carotovorum LY34 (2005), Res. Microbiol., 156, 145-153.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Pectobacterium carotovorum

Protein Variants

Protein Variants	Comment	Organism
E182A	inactive mutant	Pectobacterium carotovorum
E374A	inactive mutant	Pectobacterium carotovorum

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	5 mM, 12% inhibition	Pectobacterium carotovorum
Cu2+	5 mM, 8% inhibition	Pectobacterium carotovorum
Hg2+	5 mM, complete inhibition	Pectobacterium carotovorum
Mn2+	5 mM, 14% inhibition	Pectobacterium carotovorum
Zn2+	5 mM, 13% inhibition	Pectobacterium carotovorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	p-nitrophenyl-beta-D-glucopyranoside	pH 7.0, 40°C	Pectobacterium carotovorum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	enhances activity, at 5 mM 11% increase in activity compared to activity without added ion	Pectobacterium carotovorum
Mg2+	enhances activity, maximal hydrolysis of p-nitrophenyl-beta-D-glucopyranoside at 5 mM, 7% increase in activity compared to activity without added ion	Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	x * 53000, SDS-PAGE	Pectobacterium carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum	-	subsp. carotovorum LY34	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
565	-	-	Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-beta-D-glucoside + H2O	-	Pectobacterium carotovorum	4-methylumbelliferone + D-glucose	-	?
p-nitrophenyl-beta-D-glucopyranoside + H2O	-	Pectobacterium carotovorum	p-nitrophenol + D-glucose	-	?
p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O	-	Pectobacterium carotovorum	p-nitrophenol + D-glucose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 53000, SDS-PAGE	Pectobacterium carotovorum

Synonyms

Synonyms	Comment	Organism
AscB protein	-	Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	pH 7.0, hydrolysis of p-nitrophenyl-beta-D-glucopyranoside	Pectobacterium carotovorum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	20°C: 55% of maximal activity, 50°C: 48% of maximal activity	Pectobacterium carotovorum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	10 min, 39% loss of activity	Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Pectobacterium carotovorum

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	9	pH 4.0: about 60% of maximal activity, pH 9.0: about 30% of maximal activity	Pectobacterium carotovorum

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Release 2023.1 (January 2023)