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Literature summary for 3.2.1.86 extracted from
- Cellobiose metabolism in Aerobacter aerogenes. 3. Cleavage of cellobiose monophosphate by a phospho-beta-glucosidase (1972), J. Biol. Chem., 247, 3420-3423.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
cellobiose 6-phosphate | - |
Klebsiella aerogenes |  |
| 0.24 | - |
gentiobiose 6-phosphate | - |
Klebsiella aerogenes | |
| 0.25 | - |
cellobiitol monophosphate | - |
Klebsiella aerogenes | |
| 0.27 | - |
methyl beta-D-glucoside phosphate | - |
Klebsiella aerogenes | |
| 0.31 | - |
arbutin 6-phosphate | - |
Klebsiella aerogenes | |
| 0.5 | - |
Salicin 6-phosphate | - |
Klebsiella aerogenes | |
| 0.5 | - |
phenyl beta-D-glucoside 6-phosphate | - |
Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
sucrose density gradient centrifugation | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose 6-phosphate + H2O | Klebsiella aerogenes | - |
D-glucose 6-phosphate + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 14 fold | Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.54 | - |
- |
Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 2 months, purified enzyme, 60% loss of activity | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arbutin 6-phosphate + H2O | - |
Klebsiella aerogenes | hydroquinone + D-glucose 6-phosphate | - |
? | |
| cellobiitol monophosphate + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
| cellobiose 6-phosphate + H2O | - |
Klebsiella aerogenes | D-glucose 6-phosphate + D-glucose | - |
? | |
| gentiobiose 6-phosphate + H2O | - |
Klebsiella aerogenes | D-glucose 6-phosphate + D-glucose | - |
? | |
| methyl beta-D-glucoside monophosphate + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
| phenyl beta-D-glucoside 6-phosphate + H2O | - |
Klebsiella aerogenes | phenol + glucose 6-phosphate | - |
? | |
| salicin 6-phosphate + H2O | - |
Klebsiella aerogenes | 2-hydroxymethylphenol + D-glucose 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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