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Literature summary for 3.2.1.85 extracted from

  • Lansky, S.; Zehavi, A.; Belrhali, H.; Shoham, Y.; Shoham, G.
    Structural basis for enzyme bifunctionality - the case of Gan1D from Geobacillus stearothermophilus (2017), FEBS J., 284, 3931-3953 .
    View publication on PubMed
Search for more literature for 3.2.1.85

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5 Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
E170Q catalytic mutant Geobacillus stearothermophilus
W433A the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity Geobacillus stearothermophilus
W433M the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus W8QF82
-
-
Geobacillus stearothermophilus T-1 W8QF82
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose-6-phosphate + H2O
-
 Geobacillus stearothermophilus D-glucose-6-phosphate + D-glucose
-
 ?
cellobiose-6-phosphate + H2O
-
 Geobacillus stearothermophilus T-1 D-glucose-6-phosphate + D-glucose
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 55204, calculated from amino acid sequence Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
Gan1D the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities Geobacillus stearothermophilus

Expression

Organism Comment Expression
Geobacillus stearothermophilus the enzyme expression is upregulated by D-galactose up