Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Staphylococcus aureus |
expressed in Escherichia coli | Lactococcus lactis |
expressed in Escherichia coli | Lacticaseibacillus casei |
Protein Variants | Comment | Organism |
---|---|---|
E160Q | 1000fold reduction in activity compared to wild type | Staphylococcus aureus |
E375Q | complete loss of activity | Staphylococcus aureus |
G332R | complete loss of catalytic activity, but same behavior during purification and in affinity to antisera as wild type | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose-6-phosphate | competitive inhibition; weak inhibition | Staphylococcus aureus | |
p-nitrophenyl-beta-thiogalactoside 6-phosphate | competitive inhibition | Staphylococcus aureus | |
p-nitrophenyl-beta-thioglucoside 6-phosphate | competitive inhibition | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
0.09 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis | |
0.1 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
0.15 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
0.16 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Staphylococcus aureus | |
0.2 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
0.3 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
0.4 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis | |
0.4 | - |
p-nitrophenyl-beta-D-glucopyranoside 6 phosphate | - |
Staphylococcus aureus | |
0.6 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
0.6 | 3 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
1.3 | 1.59 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose-6-phosphate + H2O | Staphylococcus aureus | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | Lactococcus lactis | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | Lacticaseibacillus casei | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
- |
- |
Lactococcus lactis | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
- |
Lactococcus lactis |
- |
Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose-6-phosphate + H2O | - |
Staphylococcus aureus | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | - |
Lactococcus lactis | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | - |
Lacticaseibacillus casei | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Staphylococcus aureus | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactococcus lactis | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lacticaseibacillus casei | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Staphylococcus aureus | o-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lactococcus lactis | o-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lacticaseibacillus casei | o-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Staphylococcus aureus | p-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactococcus lactis | p-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lacticaseibacillus casei | p-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Staphylococcus aureus | p-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lactococcus lactis | p-nitrophenol + beta-D-glucose-6-phosphate | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | only little activity | Lacticaseibacillus casei | p-nitrophenol + beta-D-glucose-6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
4 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Staphylococcus aureus | |
4 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
5 | - |
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
7 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Lactococcus lactis | |
7 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
9 | - |
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate | - |
Staphylococcus aureus | |
18 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
22 | - |
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis | |
57 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei | |
63 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Staphylococcus aureus | |
83 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus lactis |