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Literature summary for 3.2.1.85 extracted from
- Lactose-hydrolyzing enzymes of Lactobacillus species (1972), Appl. Microbiol., 24, 51-57.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | 1.59 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lacticaseibacillus casei |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Lacticaseibacillus casei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | Lacticaseibacillus casei | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
L. casei 9595 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lacticaseibacillus casei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.9 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate, maximum of activity | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | - |
Lacticaseibacillus casei | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lacticaseibacillus casei | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-D-phosphogalactoside galactohydrolase | - |
Lacticaseibacillus casei |
| beta-Pgal | - |
Lacticaseibacillus casei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | Lacticaseibacillus casei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Lacticaseibacillus casei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Lacticaseibacillus casei |
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Release 2023.1 (January 2023)
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