BRENDA - Enzyme Database
show all sequences of 3.2.1.85

Regulation of lactose catabolism in Streptococcus mutans: purification and regulatory properties of phospho-beta-galactosidase

Calmes, R.; Brown, A.T.; Infect. Immun. 23, 68-79 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-phosphoglycerate
26% inhibition at 4.2 mM
Streptococcus mutans
ATP
100% inhibition at 2.1 mM
Streptococcus mutans
D-fructose-1,6-diphosphate
19% inhibition at 4.2 mM
Streptococcus mutans
D-fructose-6-phosphate
20% inhibition at 4.2 mM
Streptococcus mutans
D-galactose-6-phosphate
55% inhibition at 4.2 mM
Streptococcus mutans
D-Glucose-6-phosphate
38% inhibition at 4.2 mM
Streptococcus mutans
phosphoenolpyruvate
97% inhibition at 1 mM
Streptococcus mutans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Streptococcus mutans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Streptococcus mutans
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
14% inhibition at 5 mM
Streptococcus mutans
Cu2+
100% inhibition at 5 mM
Streptococcus mutans
Zn2+
57% inhibition at 5 mM
Streptococcus mutans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
strain SL1, gel filtration
Streptococcus mutans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
lactose-6-phosphate + H2O
Streptococcus mutans
-
D-glucose + beta-D-galactose-6-phosphate
-
Streptococcus mutans
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus mutans
-
-
-
Purification (Commentary)
Commentary
Organism
-
Streptococcus mutans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20.9
-
purified enzyme
Streptococcus mutans
Storage Stability
Storage Stability
Organism
-70°C, purified enzyme, at least 6 months
Streptococcus mutans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose-6-phosphate + H2O
-
208846
Streptococcus mutans
D-glucose + beta-D-galactose-6-phosphate
-
208846
Streptococcus mutans
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
208846
Streptococcus mutans
o-nitrophenol + beta-D-galactose-6-phosphate
-
208846
Streptococcus mutans
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
most active in Tris-HCl buffer
Streptococcus mutans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-phosphoglycerate
26% inhibition at 4.2 mM
Streptococcus mutans
ATP
100% inhibition at 2.1 mM
Streptococcus mutans
D-fructose-1,6-diphosphate
19% inhibition at 4.2 mM
Streptococcus mutans
D-fructose-6-phosphate
20% inhibition at 4.2 mM
Streptococcus mutans
D-galactose-6-phosphate
55% inhibition at 4.2 mM
Streptococcus mutans
D-Glucose-6-phosphate
38% inhibition at 4.2 mM
Streptococcus mutans
phosphoenolpyruvate
97% inhibition at 1 mM
Streptococcus mutans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Streptococcus mutans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Streptococcus mutans
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
14% inhibition at 5 mM
Streptococcus mutans
Cu2+
100% inhibition at 5 mM
Streptococcus mutans
Zn2+
57% inhibition at 5 mM
Streptococcus mutans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
strain SL1, gel filtration
Streptococcus mutans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
lactose-6-phosphate + H2O
Streptococcus mutans
-
D-glucose + beta-D-galactose-6-phosphate
-
Streptococcus mutans
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptococcus mutans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20.9
-
purified enzyme
Streptococcus mutans
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, purified enzyme, at least 6 months
Streptococcus mutans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lactose-6-phosphate + H2O
-
208846
Streptococcus mutans
D-glucose + beta-D-galactose-6-phosphate
-
208846
Streptococcus mutans
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
208846
Streptococcus mutans
o-nitrophenol + beta-D-galactose-6-phosphate
-
208846
Streptococcus mutans
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
most active in Tris-HCl buffer
Streptococcus mutans
Other publictions for EC 3.2.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750522
Lansky
Structural basis for enzyme b ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus T-1
FEBS J.
284
3931-3953
2017
-
-
-
1
3
-
-
-
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1
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2
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1
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2
1
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1
3
-
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1
-
-
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-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
731086
Lansky
Purification, crystallization ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus T1
Acta Crystallogr. Sect. F
70
225-231
2014
-
-
1
1
-
-
-
-
1
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
1
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1
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1
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1
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1
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1
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1
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-
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-
1
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-
1
-
-
-
-
1
1
-
-
-
732252
Honda
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323
J. Gen. Appl. Microbiol.
58
11-17
2012
-
-
1
-
-
-
-
2
-
-
1
-
-
4
-
-
1
-
-
-
-
-
8
1
2
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
2
-
-
-
-
8
1
2
-
-
2
2
-
-
-
-
1
2
-
2
2
709326
Fortina
A study of lactose metabolism ...
Lactococcus garvieae
J. Food Prot.
72
1248-1254
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
696781
Hill
Computational analysis of glyc ...
Lactococcus lactis
Biopolymers
89
1021-1031
2008
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
677688
Yu
Analysis of the mechanism and ...
Clostridium acetobutylicum
Appl. Environ. Microbiol.
73
1842-1850
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
1
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681716
Honda
beta-Galactosidase, phospho-be ...
Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus johnsonii JCM 2122, Lactobacillus mucosae
Lett. Appl. Microbiol.
45
461-466
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
1
-
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663711
Aleksandrzak-Piekarczyk
Alternative lactose catabolic ...
Lactococcus lactis
Appl. Environ. Microbiol.
71
6060-6069
2005
-
-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
655608
Thompson
Purification and some properti ...
Leptotrichia buccalis
FEMS Microbiol. Lett.
214
183-188
2002
-
-
-
-
-
-
3
1
-
1
1
1
-
3
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
1
-
1
1
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
657216
Schulte
Engineering the active center ...
Lactococcus lactis
Protein Eng.
13
515-518
2000
-
-
1
-
3
-
-
11
-
-
-
1
-
3
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
11
-
-
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208856
Wiesmann
Crystal structures and mechani ...
Lactobacillus casei, Lactococcus lactis
J. Mol. Biol.
269
851-860
1997
-
-
2
1
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
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-
2
-
1
-
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-
-
-
-
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-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208854
Suzuki
Purification and characterizat ...
Lactobacillus gasseri, Lactobacillus gasseri JCM 1031
Biosci. Biotechnol. Biochem.
60
139-141
1996
6
-
-
-
-
-
3
1
-
2
1
2
-
4
-
-
1
-
-
-
-
-
4
-
-
-
-
-
1
1
1
-
-
-
-
6
-
-
-
-
-
-
-
3
-
1
-
2
1
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
208855
Suzuki
Coexistence of two kinds of 6- ...
Lactobacillus gasseri, Lactobacillus gasseri JCM 1031
Biosci. Biotechnol. Biochem.
60
708-710
1996
2
-
-
-
-
-
4
1
2
5
-
2
-
5
-
-
1
-
-
-
-
-
4
-
2
-
1
-
1
-
1
-
-
-
-
2
-
-
-
-
-
-
-
4
-
1
2
5
-
2
-
-
-
1
-
-
-
-
4
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
208853
Staedtler
Identification of the active s ...
Staphylococcus aureus
Eur. J. Biochem.
232
658-663
1995
-
-
-
-
-
-
3
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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3
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208852
Witt
6-Phospho-beta-galactosidases ...
Lactobacillus casei, Lactococcus lactis, Staphylococcus aureus
Protein Eng.
6
913-920
1993
-
-
3
-
3
-
3
12
-
-
-
3
-
9
-
-
3
-
-
-
-
-
15
-
-
-
-
12
-
-
-
-
-
-
-
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-
3
-
-
3
-
-
3
-
12
-
-
-
3
-
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-
3
-
-
-
-
15
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
208851
Witt
A new protein that replaces th ...
Staphylococcus aureus
Protein Eng.
5
267-271
1992
-
-
1
-
1
-
1
1
-
-
-
1
-
3
-
-
1
-
-
-
1
-
2
-
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1
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1
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1
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1
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1
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1
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-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208839
Porter
Nucleotide sequence of the bet ...
Lactobacillus casei
Gene
62
263-276
1988
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
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-
1
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-
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1
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1
1
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-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
208840
Boizet
Isolation and structural analy ...
Lactococcus lactis
Gene
62
249-261
1988
-
-
1
-
-
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
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-
1
1
-
-
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-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208841
Breidt
Cloning and expression of the ...
Staphylococcus aureus
J. Bacteriol.
166
1061-1066
1986
-
-
1
-
-
-
-
-
-
-
1
1
-
7
-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
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-
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1
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1
1
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208842
Maeda
Cloning, expression and locati ...
Lactococcus lactis
J. Gen. Microbiol.
132
331-340
1986
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
2
-
2
-
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-
-
-
-
-
-
-
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-
1
-
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1
1
-
-
-
-
-
-
2
-
2
-
-
-
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208843
Jimeno
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The occurrence of beta-galacto ...
Lactobacillus casei
FEMS Microbiol. Lett.
25
275-278
1984
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208844
Lee
Cloning and expression of the ...
Lactobacillus casei
J. Bacteriol.
152
1138-1146
1982
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208845
Hall
Lactose metabolism involving p ...
Klebsiella sp., Klebsiella sp. RE 1755A
J. Bacteriol.
138
691-698
1979
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4
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208846
Calmes
Regulation of lactose cataboli ...
Streptococcus mutans
Infect. Immun.
23
68-79
1979
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3
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208847
Hengstenberg
6-phospho-beta-D-Galactosidase ...
Staphylococcus aureus
Methods Enzymol.
42
491-494
1975
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3
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208848
Johnson
beta-D-Phosphogalactoside gala ...
Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. cremoris HP
J. Bacteriol.
117
667-674
1974
2
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208849
Premi
Lactose-hydrolyzing enzymes of ...
Lactobacillus casei
Appl. Microbiol.
24
51-57
1972
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208850
Hengstenberg
Purification of the staphyloco ...
Staphylococcus aureus
Eur. J. Biochem.
14
27-32
1970
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