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Literature summary for 3.2.1.84 extracted from

  • Wiater, A.; Szczodrak, J.; Pleszczynskaa, M.
    Mutanase Induction in Trichoderma harzianum by Cell Wall of Laetiporus sulphureus and its Application for Mutan Removal from Oral Biofilms (2008), J. Microbiol. Biotechnol., 18, 1335-1341.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alpha-1,3-glucan streptococcal mutan, induction Trichoderma harzianum
cell wall material from fruiting bodies of Laetiporus sulphureus induction Trichoderma harzianum

Application

Application Comment Organism
medicine removal of oral biofilms; possible active ingredients in chewing gum, mouthwash, dental gel, toothpaste Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trichoderma harzianum
-
-

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
rich in alpha-(1-3)-glucans
-
Trichoderma harzianum F-340
-
rich in alpha-(1-3)-glucans
-

Purification (Commentary)

Purification (Comment) Organism
yield of 56.3% of alkali-soluble and water-insoluble D-glucan Trichoderma harzianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
medium supplementation with more than 0.4% cell wall preparation results in lower specific activity Trichoderma harzianum
0.178
-
after incubation for 1 h at 45°C, medium supplementation with 2.0% cell wall preparation of Laetiporus sulphureus Trichoderma harzianum
1.72
-
after incubation for 1 h at 45°C, medium supplementation with 0.4% cell wall preparation of Laetiporus sulphureus Trichoderma harzianum

Storage Stability

Storage Stability Organism
45°C, 12 h Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-glucan + H2O
-
Trichoderma harzianum alpha-D-glucose
-
?
alpha-1,3-glucan + H2O
-
Trichoderma harzianum F-340 alpha-D-glucose
-
?
sucrose + H2O
-
Trichoderma harzianum D-glucose + D-fructose
-
?
sucrose + H2O
-
Trichoderma harzianum F-340 D-glucose + D-fructose
-
?

Synonyms

Synonyms Comment Organism
mutanase
-
Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable for 12 h Trichoderma harzianum