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Literature summary for 3.2.1.84 extracted from

  • Shimotsuura, I.; Kigawa, H.; Ohdera, M.; Kuramitsu, H.K.; Nakashima, S.
    Biochemical and Molecular Characterization of a Novel Type of Mutanase from Paenibacillus sp. Strain RM: Identificatiion of its Mutan-Binding Domain, Essential for Degradation of Streptococcus mutans Biofilms (2008), Appl. Environ. Microbiol., 74, 2759-2765.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine degradation of Streptococcus mutans biofilms, treatment of dental caries Paenibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant proteins in E. coli strains NV522 an B221 Paenibacillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Paenibacillus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
by MALDI-TOF mass spectrometry Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mutan Paenibacillus sp. degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats glucan
-
?
mutan Paenibacillus sp. RM1 degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats glucan
-
?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. E16590
-
-
Paenibacillus sp. RM1 E16590
-
-

Purification (Commentary)

Purification (Comment) Organism
by chromatographic procedures Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the specific activity for degradation of Streptococcus mutans biofilms is 1.4 micromol/ml after incubation at 35°C at pH 6.0 for 3 min, degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats Paenibacillus sp.
5.6
-
incubation at 35°C for 10 min, stop reaction by adding 0.4 ml of dinitrosalicylic acid solution, N-terminal domain with mutan-binding activity, C-terminal domain with mutanase activity and only low mutan-binding activity Paenibacillus sp.

Storage Stability

Storage Stability Organism
below 60°C, 10 min Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mutan
-
Paenibacillus sp. glucan low-molecular-weight ?
mutan degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats Paenibacillus sp. glucan
-
?
mutan
-
Paenibacillus sp. RM1 glucan low-molecular-weight ?
mutan degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats Paenibacillus sp. RM1 glucan
-
?

Synonyms

Synonyms Comment Organism
mutanase
-
Paenibacillus sp.
Mutanase RM1
-
Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
above unstable, stable for 10 min Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 7 80% of residual activity in comparison to the activity at ph 4.0 Paenibacillus sp.