Application | Comment | Organism |
---|---|---|
medicine | degradation of Streptococcus mutans biofilms, treatment of dental caries | Paenibacillus sp. |
Cloned (Comment) | Organism |
---|---|
recombinant proteins in E. coli strains NV522 an B221 | Paenibacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paenibacillus sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
by MALDI-TOF mass spectrometry | Paenibacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
mutan | Paenibacillus sp. | degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats | glucan | - |
? | |
mutan | Paenibacillus sp. RM1 | degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats | glucan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | E16590 | - |
- |
Paenibacillus sp. RM1 | E16590 | - |
- |
Purification (Comment) | Organism |
---|---|
by chromatographic procedures | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity for degradation of Streptococcus mutans biofilms is 1.4 micromol/ml after incubation at 35°C at pH 6.0 for 3 min, degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats | Paenibacillus sp. |
5.6 | - |
incubation at 35°C for 10 min, stop reaction by adding 0.4 ml of dinitrosalicylic acid solution, N-terminal domain with mutan-binding activity, C-terminal domain with mutanase activity and only low mutan-binding activity | Paenibacillus sp. |
Storage Stability | Organism |
---|---|
below 60°C, 10 min | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mutan | - |
Paenibacillus sp. | glucan | low-molecular-weight | ? | |
mutan | degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats | Paenibacillus sp. | glucan | - |
? | |
mutan | - |
Paenibacillus sp. RM1 | glucan | low-molecular-weight | ? | |
mutan | degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats | Paenibacillus sp. RM1 | glucan | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mutanase | - |
Paenibacillus sp. |
Mutanase RM1 | - |
Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
above unstable, stable for 10 min | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Paenibacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | 80% of residual activity in comparison to the activity at ph 4.0 | Paenibacillus sp. |