Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 180% activity at 2 mM | Bacillus sp. HT19 | |
Cu2+ | about 110% activity at 2 mM | Bacillus sp. HT19 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | about 80% residual activity at 2 mM | Bacillus sp. HT19 | |
EDTA | about 90% residual activity at 2 mM | Bacillus sp. HT19 | |
Fe2+ | about 70% residual activity at 2 mM | Bacillus sp. HT19 | |
Sr2+ | about 50% residual activity at 2 mM | Bacillus sp. HT19 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ni2+, Mg2+ and Mn2+ have almost no effect on the enzyme activity | Bacillus sp. HT19 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | Bacillus sp. HT19 | - |
kappa-neocarrabiose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. HT19 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Sephadex G200 gel filtration | Bacillus sp. HT19 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | - |
Bacillus sp. HT19 | kappa-neocarrabiose | - |
? | |
additional information | no activity with iota-carrageenan, lambda-carrageenan, agar, and sodium alginate | Bacillus sp. HT19 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Bacillus sp. HT19 |
Synonyms | Comment | Organism |
---|---|---|
Car19 | - |
Bacillus sp. HT19 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. HT19 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | about 40% activity at 40°C, about 90% activity at 50°C, 100% activity at 60°C, about 55% activity at 70°C | Bacillus sp. HT19 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme retains more than 90% of its initial activity after incubation at 50-60°C for 24 h. The activity declines sharply after 60 min of incubation at 70°C | Bacillus sp. HT19 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus sp. HT19 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 65% activity between pH 6.0 and 9.0 | Bacillus sp. HT19 |