Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.83 extracted from

  • Wang, L.; Li, S.; Zhang, S.; Li, J.; Yu, W.; Gong, Q.
    A new kappa-carrageenase CgkS from marine bacterium Shewanella sp. Kz7 (2015), J. Ocean Univ. China, 14, 759-763 .
No PubMed abstract available
Search for more literature for 3.2.1.83

Activating Compound

Activating Compound Comment Organism Structure
NaCl the enzyme does not require NaCl for activity, although its activity is enhanced by NaCl (226.8% activity at 200 mM) Shewanella sp. Kz7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Shewanella sp. Kz7

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 72.3% residual activity at 1 mM Shewanella sp. Kz7
Cu2+ 26.3% residual activity at 1 mM Shewanella sp. Kz7
EDTA 90.8% residual activity at 1 mM Shewanella sp. Kz7
Fe2+ 83.8% residual activity at 1 mM Shewanella sp. Kz7
K+ 82.6% residual activity at 1 mM Shewanella sp. Kz7
Li+ 92.1% residual activity at 1 mM Shewanella sp. Kz7
Mg2+ 83.3% residual activity at 1 mM Shewanella sp. Kz7
NH4+ 81.1% residual activity at 1 mM Shewanella sp. Kz7
SDS 21.1% residual activity at 1 mM Shewanella sp. Kz7
Zn2+ 35.4% residual activity at 1 mM Shewanella sp. Kz7

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 132.4% activity at 1 mM Shewanella sp. Kz7
Mn2+ 109.2% activity at 1 mM Shewanella sp. Kz7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Shewanella sp. Kz7
-
 kappa-neocarratetraose + kappa-neocarrabiose
-
 ?

Organism

Organism UniProt Comment Textmining
Shewanella sp. Kz7 W8V7X4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Shewanella sp. Kz7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
-
 Shewanella sp. Kz7 kappa-neocarratetraose + kappa-neocarrabiose
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Shewanella sp. Kz7
? x * 42743, calculated from amino acid sequence Shewanella sp. Kz7

Synonyms

Synonyms Comment Organism
CgkS
-
 Shewanella sp. Kz7

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Shewanella sp. Kz7

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 85% of the enzymatic activities remain after being incubated at 30°C for 1 h Shewanella sp. Kz7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in phosphate buffer Shewanella sp. Kz7

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the enzyme is stable within a range of pH 6.0-9.0 Shewanella sp. Kz7

pI Value

Organism Comment pI Value Maximum pI Value
Shewanella sp. Kz7 calculated from amino acid sequence
-
 9.1