Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.83 extracted from

  • Zhu, B.; Ning, L.
    Purification and characterization of a new kappa-carrageenase from the marine bacterium Vibrio sp. NJ-2 (2016), J. Microbiol. Biotechnol., 26, 255-262 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 98% residual activity at 1 mM Vibrio sp. NJ-2
Cu2+ 32% residual activity at 1 mM Vibrio sp. NJ-2
EDTA 71% residual activity at 1 mM Vibrio sp. NJ-2
Fe2+ 51% residual activity at 1 mM Vibrio sp. NJ-2
Fe3+ 38% residual activity at 1 mM Vibrio sp. NJ-2
Mn2+ 39% residual activity at 1 mM Vibrio sp. NJ-2
Ni2+ 49% residual activity at 1 mM Vibrio sp. NJ-2
SDS 56% residual activity at 1 mM Vibrio sp. NJ-2
Zn2+ 21% residual activity at 1 mM Vibrio sp. NJ-2

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 108% activity at 1 mM Vibrio sp. NJ-2
K+ 153% activity at 1 mM Vibrio sp. NJ-2
Mg2+ 112% activity at 1 mM Vibrio sp. NJ-2
Na+ 369% activity at 300 mM Vibrio sp. NJ-2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Vibrio sp. NJ-2
-
kappa-neocarraoctaose-sulfate + kappa-neocarrahexaose-sulfate + kappa-neocarratetraose-sulfate + kappa-neocarrabiose-sulfate
-
?

Organism

Organism UniProt Comment Textmining
Vibrio sp. NJ-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex 200 gel filtration, and Sephadex G-75 gel filtration Vibrio sp. NJ-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
-
Vibrio sp. NJ-2 kappa-neocarraoctaose-sulfate + kappa-neocarrahexaose-sulfate + kappa-neocarratetraose-sulfate + kappa-neocarrabiose-sulfate
-
?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Vibrio sp. NJ-2

Synonyms

Synonyms Comment Organism
CgkA
-
Vibrio sp. NJ-2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Vibrio sp. NJ-2

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 the enzyme maintains more than 60% of total activity at 30-50°C Vibrio sp. NJ-2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 the enzyme maintains more than 90% of total activity after 30 min at 10-40°C. At 50°C, the activity drops to about 20% Vibrio sp. NJ-2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Vibrio sp. NJ-2

pH Range

pH Minimum pH Maximum Comment Organism
6 9 the enzyme maintains more than 70% of total activity between pH 6.0 and 9.0 Vibrio sp. NJ-2

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 the enzyme maintains 80% of total activity after 24 h between pH 6.0 and 10.0. At pH 5.0 and 11.0, the activity drops to about 55% and 2%, respectively Vibrio sp. NJ-2