Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | highest activity at 100 mM | Wenyingzhuangia aestuarii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Wenyingzhuangia aestuarii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 68.27% residual activity at 1 mM | Wenyingzhuangia aestuarii | |
Hg2+ | 15.35% residual activity at 1 mM | Wenyingzhuangia aestuarii | |
Mn2+ | 77.66% residual activity at 1 mM | Wenyingzhuangia aestuarii | |
SDS | 6.4% residual activity at 1 mM | Wenyingzhuangia aestuarii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
kappa-carrageen | at pH 8.0 and 25°C | Wenyingzhuangia aestuarii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 133.23% activity at 1 mM | Wenyingzhuangia aestuarii | |
Cd2+ | 117.9% activity at 1 mM | Wenyingzhuangia aestuarii | |
additional information | the enzyme is not significantly influenced by K+, Mg2+ and Zn2+ | Wenyingzhuangia aestuarii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | Wenyingzhuangia aestuarii | - |
kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
kappa-carrageen + H2O | Wenyingzhuangia aestuarii OF219 | - |
kappa-neocarratetraose + kappa-neocarrabiose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wenyingzhuangia aestuarii | A0A223FUT7 | - |
- |
Wenyingzhuangia aestuarii OF219 | A0A223FUT7 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Wenyingzhuangia aestuarii |
Storage Stability | Organism |
---|---|
4°C, purified enzyme without any protective additives, at least 30 days, no loss of activity | Wenyingzhuangia aestuarii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | - |
Wenyingzhuangia aestuarii | kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
kappa-carrageen + H2O | - |
Wenyingzhuangia aestuarii OF219 | kappa-neocarratetraose + kappa-neocarrabiose | - |
? | |
additional information | the enzyme is incapable of hydrolyzing iota-carrageenan, lambda-carrageenan and agarose | Wenyingzhuangia aestuarii | ? | - |
? | |
additional information | the enzyme is incapable of hydrolyzing iota-carrageenan, lambda-carrageenan and agarose | Wenyingzhuangia aestuarii OF219 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, recombinant enzyme, SDS-PAGE | Wenyingzhuangia aestuarii |
? | x * 66582, calculated from amino acid sequence | Wenyingzhuangia aestuarii |
Synonyms | Comment | Organism |
---|---|---|
Cgk16A | - |
Wenyingzhuangia aestuarii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Wenyingzhuangia aestuarii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 30 | the enzyme shows 81% and 52% of its maximal activity at 20°C and 15°C respectively. The activity vanishes rapidly at 30°C and 40°C | Wenyingzhuangia aestuarii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
42% of residual activity is retained after 24 h at 25°C | Wenyingzhuangia aestuarii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.54 | - |
kappa-carrageen | at pH 8.0 and 25°C | Wenyingzhuangia aestuarii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Wenyingzhuangia aestuarii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | the enzyme is stable for 1 h between pH 6.0-10.5 | Wenyingzhuangia aestuarii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
165630 | - |
kappa-carrageen | at pH 8.0 and 25°C | Wenyingzhuangia aestuarii |