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Literature summary for 3.2.1.83 extracted from

  • Kang, D.H.; Hyeon, J.E.; You, S.K.; Kim, S.W.; Han, S.O.
    Efficient enzymatic degradation process for hydrolysis activity of the carrageenan from red algae in marine biomass (2014), J. Biotechnol., 192, 108-113.
    View publication on PubMed
Search for more literature for 3.2.1.83

Cloned(Commentary)

Cloned (Comment) Organism
gene cgkA, recombinant expression of functional CBM-tagged chimeric fusion enzyme cCgkA-cCglA, consisting of the catalytic domains of kappa-carrageenase CgkA and lambda-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain, in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha Pseudoalteromonas carrageenovora

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric fusion protein consisting of the catalytic domains of kappa-carrageenase CgkA and lambda-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain from Clostridium cellulovorans. The recombinant cCgkA and cCglA are assembled with scaffoldin miniCbpA from Clostridium cellulovorans via cohesin and dockerin interaction. Carbohydrate binding module in scaffoldin is used as a tag Pseudoalteromonas carrageenovora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, catalytic domain of kappa-carrageenase CgkA, SDS-PAGE Pseudoalteromonas carrageenovora

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas carrageenovora P43478 gene cgkA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CBM-tagged chimeric fusion enzyme cCgkA-cCglA by cellulose affinity purification using cellulose as a support or by nickel affinity chromatography Pseudoalteromonas carrageenovora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
-
 Pseudoalteromonas carrageenovora ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, catalytic domain of kappa-carrageenase CgkA, SDS-PAGE Pseudoalteromonas carrageenovora

Synonyms

Synonyms Comment Organism
CgkA
-
 Pseudoalteromonas carrageenovora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Pseudoalteromonas carrageenovora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudoalteromonas carrageenovora