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Literature summary for 3.2.1.83 extracted from

  • Liu, Z.; Tian, L.; Chen, Y.; Mou, H.
    Efficient extracellular production of kappa-carrageenase in Escherichia coli: effects of wild-type signal sequence and process conditions on extracellular secretion (2014), J. Biotechnol., 185, 8-14.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene cgkZ, recombinant expression of N-terminally His6-tagged extracellular enzyme in Escherichia coli strain BL21(DE3), 51% of the total kappa-carrageenase is secreted into culture medium, and 33% accumulates in the periplasmic space. Low temperature of 23°C and optimal isopropyl-beta-L-thiogalactoside concentration of 0.9 mM favor soluble protein expression, additives such as lactose, glycine, Tween-80, and Triton X-100 promote the release of intracellular enzymes, method optimization and evaluation, overview Zobellia sp. M-2

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme contains a 28-amino acid signal peptide Zobellia sp. M-2
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Zobellia sp. M-2
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 ?
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 ?

Organism

Organism UniProt Comment Textmining
Zobellia sp. M-2 S5U3X4 i.e. strain ZM-2, gene cgkZ
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
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 Zobellia sp. M-2 ?
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 ?

Synonyms

Synonyms Comment Organism
CgkZ
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 Zobellia sp. M-2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
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 assay at Zobellia sp. M-2

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 16, GH16 Zobellia sp. M-2