KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for kappa-carrageenan is 1.647 mg/ml at 35°C and pH 7.0 | Cellulophaga lytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40800 | - |
x * 40800, SDS-PAGE | Cellulophaga lytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulophaga lytica | - |
isolated from the sediment of carrageenan production base in Hainan (Hainan, China) | - |
Cellulophaga lytica N5-2 | - |
isolated from the sediment of carrageenan production base in Hainan (Hainan, China) | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme to electrophoretic homogeneity from culture supernatant by ammonium sulfate fractionation, dialysis, and two different steps of gel filtration | Cellulophaga lytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1170 | - |
purified enzyme, pH 7.0, 35°C | Cellulophaga lytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | the enzyme is specific for the beta-1,4 linkage and hydrolyzes kappa-carrageenan into kappa-neocarraoctaose-sulfate and kappa-neocarrahexaose-sulfate first, and then brakes kappa-neocarraoctaose-sulfate into kappa-neocarrabiose-sulfate and kappa-neocarrahexaose-sulfate, NMR spectroscopy analysis, overview | Cellulophaga lytica | kappa-neocarrahexaose-sulfate + kappa-neocarrabiose-sulfate + kappa-neocarrahexaose-sulfate | - |
? | |
kappa-carrageen + H2O | the enzyme is specific for the beta-1,4 linkage and hydrolyzes kappa-carrageenan into kappa-neocarraoctaose-sulfate and kappa-neocarrahexaose-sulfate first, and then brakes kappa-neocarraoctaose-sulfate into kappa-neocarrabiose-sulfate and kappa-neocarrahexaose-sulfate, NMR spectroscopy analysis, overview | Cellulophaga lytica N5-2 | kappa-neocarrahexaose-sulfate + kappa-neocarrabiose-sulfate + kappa-neocarrahexaose-sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40800, SDS-PAGE | Cellulophaga lytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Cellulophaga lytica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range | Cellulophaga lytica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, pH 7.0, 2.5 h, stable at | Cellulophaga lytica |
50 | - |
purified enzyme, pH 7.0, inactivation within 90 min | Cellulophaga lytica |
60 | - |
purified enzyme, pH 7.0, inactivation within 60 min | Cellulophaga lytica |
70 | - |
purified enzyme, pH 7.0, inactivation within 30 min | Cellulophaga lytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cellulophaga lytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | activity range | Cellulophaga lytica |