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Literature summary for 3.2.1.83 extracted from
- Physical state of kappa-carrageenan modulates the mode of action of kappa-carrageenase from Pseudoalteromonas carrageenovora (2009), Biochem. J., 419, 545-553.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed as a His-tagged fusion protein in Escherichia coli | Pseudoalteromonas carrageenovora |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas carrageenovora | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by metal-affinity chromatography followed by cation exchange chromatography | Pseudoalteromonas carrageenovora |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.2 M Tris-HCl buffer | Pseudoalteromonas carrageenovora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageenan + H2O | the average molecular mass of solid carrageenan decreases very slowly, and tetrasaccharide production is high. kappa-carrageenase is a processive enzyme when acting on solid substrates. It has a random mode of action on soluble substrates. The conditions for processivity not only require that the active site possesses the adapted topology, but also that the substrate is in an appropriate physical and conformational state (processivity is similar for gel and powder) | Pseudoalteromonas carrageenovora | ? | - |
? |  |
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