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Literature summary for 3.2.1.81 extracted from
- Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp. CY24 (2010), Acta Crystallogr. Sect. F, 66, 1635-1639.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudoalteromonas sp. |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.0, 30% PEG 4000, 0.1 M ammonium sulfate and 15% glycerol | Pseudoalteromonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-affinity column chromatography, HiTrap DEAE column chromatography, and Superdex 200 gel filtration | Pseudoalteromonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | AgaB hydrolyzes the glycosylic bond with inversion of anomeric configuration | Pseudoalteromonas sp. | neoagarooctaose + neoagarodecaose | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaB | - |
Pseudoalteromonas sp. |
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