Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | beta-mercaptoethanol even at a low concentration of 10 mM increases the activity of AgaA by 23% | Agarivorans sp. LQ48 | |
dithiothreitol | dithiothreitol even at a low concentration of 10 mM increases the activity of AgaA by 21% | Agarivorans sp. LQ48 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Agarivorans sp. LQ48 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-(2-nitrobenzoic acid) | 53% residual activity at 10 mM | Agarivorans sp. LQ48 | |
Cu2+ | 69% residual activity at 10 mM | Agarivorans sp. LQ48 | |
EDTA | 69% residual activity at 10 mM | Agarivorans sp. LQ48 | |
Mg2+ | 65% residual activity at 10 mM | Agarivorans sp. LQ48 | |
SDS | 71% residual activity at 10 mM | Agarivorans sp. LQ48 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | low concentration of Mn2+ have a slight positive effect on the AgaA activity (116% relative activity at 1 mM) | Agarivorans sp. LQ48 | |
additional information | no significant activation or inhibition of AgaA is observed by Na+, K+, Ca2+, Fe3+, and urea | Agarivorans sp. LQ48 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47200 | - |
SDS-PAGE | Agarivorans sp. LQ48 |
51200 | - |
calculated from amino acid sequence | Agarivorans sp. LQ48 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarivorans sp. LQ48 | C9WIW7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Agarivorans sp. LQ48 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | the recombinant enzyme hydrolyzes the beta-1,4-glycosidic linkages of agarose, yielding neoagarotetraose and neoagarohexaose as the main products | Agarivorans sp. LQ48 | neoagarotetraose + neoagarohexaose | - |
? | |
additional information | when neoagarotetraose and neoagarohexaose are used as substrates for AgaA, no hydrolysis products are detected | Agarivorans sp. LQ48 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AgaA | - |
Agarivorans sp. LQ48 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Agarivorans sp. LQ48 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Agarivorans sp. LQ48 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | AgaA still retains more than 95% activity after incubation at pH 3.0-11.0 for 1 h | Agarivorans sp. LQ48 |