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Literature summary for 3.2.1.81 extracted from

  • Hehemann, J.H.; Michel, G.; Barbeyron, T.; Czjzek, M.
    Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a beta-agarase from the flavobacterium Zobellia galactanivorans (2010), Acta Crystallogr. Sect. F, 66, 413-417.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Zobellia galactanivorans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 28-34% (w/v) PEG 8000 and 0.1 M imidazole pH 8.0, at 19°C Zobellia galactanivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Zobellia galactanivorans
-
neoagarotetraose
-
?

Organism

Organism UniProt Comment Textmining
Zobellia galactanivorans Q9RGX8 formerly Cytophaga drobachiensis
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity column chromatography and Sephacryl S-200 gel filtration Zobellia galactanivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
Zobellia galactanivorans neoagarotetraose
-
?

Synonyms

Synonyms Comment Organism
AgaD
-
Zobellia galactanivorans
beta-agarase D
-
Zobellia galactanivorans