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Literature summary for 3.2.1.81 extracted from

  • Fu, X.T.; Pan, C.H.; Lin, H.; Kim, S.M.
    Gene cloning, expression, and characterization of a beta-agarase, AgaB34, from Agarivorans albus YKW-34 (2009), J. Microbiol. Biotechnol., 19, 257-264.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation of rAgaB34 by chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea) Agarivorans albus

Cloned(Commentary)

Cloned (Comment) Organism
DNA fragments (3.0-8.0 kb) ligated into vector pUC18 and transformed into Escherichia coli DH5alpha. Vector pUSH1, which produces a derivative of rAgaB34 with a 6 × His tag at its C-terminal, transformed into Escherichia coli DH5alpha Agarivorans albus

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
Agarivorans albus
Cu2+
-
Agarivorans albus
Mn2+
-
Agarivorans albus
additional information no inhibition of rAgaB34 by metal ions Na+, K+, Mg2+, Ca2+, and Ba2+, chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea) Agarivorans albus
Zn2+
-
Agarivorans albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
agarose
-
Agarivorans albus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation of rAgaB34 by Na+, K+, Mg2+, Ca2+, and Ba2+ Agarivorans albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
x * 49000, SDS-PAGE Agarivorans albus

Organism

Organism UniProt Comment Textmining
Agarivorans albus A8W969
-
-
Agarivorans albus YKW-34 A8W969
-
-

Purification (Commentary)

Purification (Comment) Organism
rAgaB34 purified by ultrafiltration and on Ni+ column, 15fold with a yield of 80% Agarivorans albus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.9
-
culture fluid Agarivorans albus
242.2
-
15fold purified rAgaB34, with agarose as substrate at 40°C Agarivorans albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
Agarivorans albus neoagarohexaose + neoagarotetraose
-
?
agarose + H2O
-
Agarivorans albus YKW-34 neoagarohexaose + neoagarotetraose
-
?
additional information neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time Agarivorans albus ?
-
?
additional information neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time Agarivorans albus YKW-34 ?
-
?
neoagarohexaose + H2O
-
Agarivorans albus neoagarotetraose + neoagarobiose
-
?
neoagarohexaose + H2O
-
Agarivorans albus YKW-34 neoagarotetraose + neoagarobiose
-
?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Agarivorans albus

Synonyms

Synonyms Comment Organism
AgaB34
-
Agarivorans albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Agarivorans albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
-
Agarivorans albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41
-
agarose
-
Agarivorans albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Agarivorans albus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
Agarivorans albus