Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation of rAgaB34 by chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea) | Agarivorans albus |
Cloned (Comment) | Organism |
---|---|
DNA fragments (3.0-8.0 kb) ligated into vector pUC18 and transformed into Escherichia coli DH5alpha. Vector pUSH1, which produces a derivative of rAgaB34 with a 6 × His tag at its C-terminal, transformed into Escherichia coli DH5alpha | Agarivorans albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | - |
Agarivorans albus | |
Cu2+ | - |
Agarivorans albus | |
Mn2+ | - |
Agarivorans albus | |
additional information | no inhibition of rAgaB34 by metal ions Na+, K+, Mg2+, Ca2+, and Ba2+, chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea) | Agarivorans albus | |
Zn2+ | - |
Agarivorans albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
agarose | - |
Agarivorans albus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation of rAgaB34 by Na+, K+, Mg2+, Ca2+, and Ba2+ | Agarivorans albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, SDS-PAGE | Agarivorans albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarivorans albus | A8W969 | - |
- |
Agarivorans albus YKW-34 | A8W969 | - |
- |
Purification (Comment) | Organism |
---|---|
rAgaB34 purified by ultrafiltration and on Ni+ column, 15fold with a yield of 80% | Agarivorans albus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.9 | - |
culture fluid | Agarivorans albus |
242.2 | - |
15fold purified rAgaB34, with agarose as substrate at 40°C | Agarivorans albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Agarivorans albus | neoagarohexaose + neoagarotetraose | - |
? | |
agarose + H2O | - |
Agarivorans albus YKW-34 | neoagarohexaose + neoagarotetraose | - |
? | |
additional information | neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time | Agarivorans albus | ? | - |
? | |
additional information | neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time | Agarivorans albus YKW-34 | ? | - |
? | |
neoagarohexaose + H2O | - |
Agarivorans albus | neoagarotetraose + neoagarobiose | - |
? | |
neoagarohexaose + H2O | - |
Agarivorans albus YKW-34 | neoagarotetraose + neoagarobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Agarivorans albus |
Synonyms | Comment | Organism |
---|---|---|
AgaB34 | - |
Agarivorans albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Agarivorans albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Agarivorans albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41 | - |
agarose | - |
Agarivorans albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Agarivorans albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Agarivorans albus |