Cloned (Comment) | Organism |
---|---|
ligated into a pET22b(+) vector and expressed in Escherichia coli BL21(DE3) competent cells | Vibrio sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | partially inhibits the activity | Vibrio sp. | |
AlCl3 | completely inhibits the activity | Vibrio sp. | |
CuCl2 | completely inhibits the activity | Vibrio sp. | |
CuSO4 | completely inhibits the activity | Vibrio sp. | |
FeCl3 | partially inhibits the activity | Vibrio sp. | |
HgCl2 | completely inhibits the activity | Vibrio sp. | |
MnCl2 | partially inhibits the activity | Vibrio sp. | |
additional information | NaCl, KCl, MgCl2, and BaCl2 negligibly affect the enzymatic activity | Vibrio sp. | |
Pb(CH3COO)2 | completely inhibits the activity | Vibrio sp. | |
SDS | 0.1% SDS completely inhibits the activity | Vibrio sp. | |
ZnCl2 | completely inhibits the activity | Vibrio sp. | |
ZnSO4 | completely inhibits the activity | Vibrio sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | slightly enhances enzymatic activity | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106100 | - |
sequence analysis | Vibrio sp. |
120000 | - |
1 * 120000, SDS-PAGE | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
18.8fold and with a final yield of 16.8% by anion exchange and hydrophobic chromatographies | Vibrio sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.872 | - |
cell lysate | Vibrio sp. |
16.4 | - |
18.8fold purified enzyme | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
agarose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
additional information | does not act on neoagarotetraose and neoagarobiose and kappa-carrageenan | Vibrio sp. | ? | - |
? | |
neoagarohexaose + H2O | - |
Vibrio sp. | ? | - |
? | |
neoagarooctaose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose + neoagarobiose | - |
? | |
porphyran + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 120000, SDS-PAGE | Vibrio sp. |
Synonyms | Comment | Organism |
---|---|---|
AgaA | - |
Vibrio sp. |
beta-agarase | - |
Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vibrio sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable under 37°C after a incubation of 10 min | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 11 | after preincubated at various pHs at 25°C for 12 h | Vibrio sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vibrio sp. | - |
- |
4.14 |