Cloned (Comment) | Organism |
---|---|
ligated into pBluescript II KS(+), and transferred into Escherichia coli DH5alpha, forming a genomic DNA library. Cloned into the pET-24a(+) vector and transformed into Escherichia coli BL21 (DE3) competent cells | Pseudoalteromonas sp. CY24 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | slightly inhibits | Pseudoalteromonas sp. CY24 | |
EDTA | slightly inhibits | Pseudoalteromonas sp. CY24 | |
Hg2+ | completely inhibits | Pseudoalteromonas sp. CY24 | |
Mn2+ | slightly inhibits | Pseudoalteromonas sp. CY24 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.36 | - |
neoagarotetradecaose | - |
Pseudoalteromonas sp. CY24 | |
12.39 | - |
neoagarododecaose | - |
Pseudoalteromonas sp. CY24 | |
12.81 | - |
neoagarodecaose | - |
Pseudoalteromonas sp. CY24 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | slightly increases activity | Pseudoalteromonas sp. CY24 | |
Ca2+ | slightly increases activity | Pseudoalteromonas sp. CY24 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50400 | - |
1 * 50400, SDS-PAGE | Pseudoalteromonas sp. CY24 |
50900 | - |
sequence analysis | Pseudoalteromonas sp. CY24 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. CY24 | A1A3Y9 | - |
- |
Purification (Comment) | Organism |
---|---|
476fold and with a final yield of 21.2%, by ammonium sulfate precipitation, weak anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Pseudoalteromonas sp. CY24 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5000 | - |
recombinant AgaB 476fold purified | Pseudoalteromonas sp. CY24 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Pseudoalteromonas sp. CY24 | neoagarooctaose + neoagarodecaose | - |
? | |
additional information | the agarase AgaB appears to have a large substrate binding cleft that accommodates 12 sugar units, with 8 sugar units toward the reducing end spanning subsites +1 to +8 and 4 sugar units toward the non-reducing end spanning subsites -4 to -1, and enzymatic cleavage taking place between subsites -1 and +1. The enzyme hydrolyzes the glycosidic bond with inversion of anomeric configuration. AgaB does not hydrolyze neoagarooctaose, kappa-carrageenan, lota-carrageenan, lambda-carrageenan, alginate, and chitosan | Pseudoalteromonas sp. CY24 | ? | - |
? | |
neoagarodecaose + H2O | is a very poor substrate | Pseudoalteromonas sp. CY24 | ? | - |
? | |
neoagarododecaose + H2O | - |
Pseudoalteromonas sp. CY24 | ? | - |
? | |
neoagarotetradecaose + H2O | - |
Pseudoalteromonas sp. CY24 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50400, SDS-PAGE | Pseudoalteromonas sp. CY24 |
Synonyms | Comment | Organism |
---|---|---|
AgaB | - |
Pseudoalteromonas sp. CY24 |
beta-agarase | - |
Pseudoalteromonas sp. CY24 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudoalteromonas sp. CY24 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
retains more than 90% activity until 35°C | Pseudoalteromonas sp. CY24 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
neoagarodecaose | - |
Pseudoalteromonas sp. CY24 | |
4.77 | - |
neoagarododecaose | - |
Pseudoalteromonas sp. CY24 | |
5.16 | - |
neoagarotetradecaose | - |
Pseudoalteromonas sp. CY24 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudoalteromonas sp. CY24 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.7 | 10.6 | retains more than 95% activity | Pseudoalteromonas sp. CY24 |