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Literature summary for 3.2.1.81 extracted from

  • Dong, J.; Hashikawa, S.; Konishi, T.; Tamaru, Y.; Araki, T.
    Cloning of the novel gene encoding beta-agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product (2006), Appl. Environ. Microbiol., 72, 6399-6401.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
PCR product cloned into a pT7Blue-2 vector. Amplified DNA fragment digested with NdeI and XhoI and ligated into a pET22b(+) vector and expressed in Escherichia coli BL21(DE3) Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 1 * 47000, SDS-PAGE Vibrio sp.
47240
-
 sequence analysis Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp. Q0KKW9
-
-

Purification (Commentary)

Purification (Comment) Organism
17.9fold with a final yield of 23.3% Vibrio sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
329
-
 recombinant AgaC Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O
-
 Vibrio sp. neoagarooctaose + neoagarohexaose + neoagarotetraose
-
 ?
agarose + H2O rAgaC with low activity (0.05 U/ml) cleaves agarose into longer neoagarooligosaccharides. When activity is increased to 30 U/ml, neoagarooctaose is produced predominantly in addition to neoagarohexaose and neoagarotetraose Vibrio sp. 2 neoagarooligosaccharides
-
 ?
additional information cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides nor kappa-carrageenan Vibrio sp. ?
-
 ?
neoagarododecaose + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose + neoagarooctaose
-
 ?
porphyran + H2O
-
 Vibrio sp. neoagarooctaose + neoagarohexaose + neoagarotetraose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Vibrio sp.

Synonyms

Synonyms Comment Organism
AgaC
-
 Vibrio sp.
beta-agarase C
-
 Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable under 37°C Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Vibrio sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
 Vibrio sp.