Literature summary for 3.2.1.81 extracted from

Allouch, J.; Helbert, W.; Henrissat, B.; Czjzek, M.
Parallel substrate binding sites in a beta-agarase suggest a novel mode of action on double-helical agarose (2004), Structure, 12, 623-632.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli XL21 Blue and ORIGAMI (DE3) pLysS cells	Zobellia galactanivorans

Crystallization (Commentary)

Crystallization (Comment)	Organism
E147S mutant in complex with agaro-octaose, 1.7 A resolution, structure determination by molecular replacment, hanging drop vapor duffusion method, 20.5°C, 30% PEG 4000, 200 mM ammonium acetate, 100 mM sodium acetate	Zobellia galactanivorans

Protein Variants

Protein Variants	Comment	Organism
E147S	inactive mutant	Zobellia galactanivorans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Zobellia galactanivorans	G0L322	Dsij	-
Zobellia galactanivorans Dsij	G0L322	Dsij	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTa affinity chromatography and exclusion chromatography with Sephacryl S100	Zobellia galactanivorans

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Release 2023.1 (January 2023)