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Literature summary for 3.2.1.81 extracted from

  • Ohta, Y.; Hatada, Y.; Ito, S.; Horikoshi, K.
    High-level expression of a neoagarobiose-producing beta-agarase gene from Agarivorans sp. JAMB-A11 in Bacillus subtilis and enzymic properties of the recombinant enzyme (2005), Biotechnol. Appl. Biochem., 41, 183-191.
    View publication on PubMed
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Agarivorans sp.

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, complete inhibition Agarivorans sp.
additional information strong resistance to Cu2+, Pb2+ and Zn2+ at 1 mM, iodoacetamide, p-chloromercuribenzoate, dithiothreitol and 2-mercaptoethanol, SDS at 30 mM Agarivorans sp.
N-bromosuccinimide 0.1 mM Agarivorans sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, stimulates enzyme Agarivorans sp.
Na+ 0.1 M, stimulates enzyme Agarivorans sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 SDS-PAGE Agarivorans sp.

Organism

Organism UniProt Comment Textmining
Agarivorans sp.
-
-
-
Agarivorans sp. JAMB-A11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m, purification by ammonium sulfate precipitation, dialysis, DEAE-Toyopearl 650M column, dialysis Agarivorans sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m Agarivorans sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
371
-
 50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 7.8, containing 10 mM CaCl2, 0.1 mM NaCl, 40°C Agarivorans sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Agarivorans sp. agarotetraose + ?
-
 ?
agarose + H2O
-
 Agarivorans sp. JAMB-A11 agarotetraose + ?
-
 ?
neoagarotetraose + H2O
-
 Agarivorans sp. neoagarobiose
-
 ?
neoagarotetraose + H2O
-
 Agarivorans sp. JAMB-A11 neoagarobiose
-
 ?

Synonyms

Synonyms Comment Organism
RagaA11
-
 Agarivorans sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 7.8, containing 10 mM CaCl2, 0.1 mM NaCl Agarivorans sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 retaining 85% of original activity after 15 min in 50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer containing 0.1 M NaCl and 10 mM CaCl2 at pH 7.8 Agarivorans sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 Britton-Robinson universal buffers, MOPS and N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer Agarivorans sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 retaining 70% of original activity Agarivorans sp.