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Literature summary for 3.2.1.81 extracted from
- Characterization of a novel beta-agarase from marine Alteromonas sp. SY37-12 and its degrading products (2005), Appl. Microbiol. Biotechnol., 71, 833-839.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | maximum activity in presence of 2% NaCl | Alteromonas sp. |  |
| NaCl | optimum activity in the presence of 2% (w/v) NaCl | Alteromonas sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Alteromonas sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39500 | - |
SDS-PAGE | Alteromonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | Alteromonas sp. | - |
neoagarotetraose + neoagarohexaose | main products | ? | |
| agar + H2O | Alteromonas sp. SY37-12 | - |
neoagarotetraose + neoagarohexaose | main products | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alteromonas sp. | - |
- |
- |
| Alteromonas sp. | - |
SY37-12 | - |
| Alteromonas sp. SY37-12 | - |
- |
- |
| Alteromonas sp. SY37-12 | - |
SY37-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alteromonas sp. |
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-100HR gel filtration | Alteromonas sp. |
| ammonium sulfate precipitation, gel filtration on Sephacryl S-100HR, ion exchange chromatography on diethylaminoethyl-Sepharose | Alteromonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | - |
cell-free medium, at pH 7.0 and 35°C | Alteromonas sp. |
| 83.5 | - |
35°C, pH 7.0 | Alteromonas sp. |
| 83.5 | - |
after 27.8fold purification, at pH 7.0 and 35°C | Alteromonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | - |
Alteromonas sp. | neoagarotetraose + neoagarohexaose | - |
? | |
| agar + H2O | - |
Alteromonas sp. | neoagarotetraose + neoagarohexaose | main products | ? | |
| agar + H2O | - |
Alteromonas sp. SY37-12 | neoagarotetraose + neoagarohexaose | - |
? | |
| agar + H2O | - |
Alteromonas sp. SY37-12 | neoagarotetraose + neoagarohexaose | main products | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39500, SDS-PAGE | Alteromonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agarase | - |
Alteromonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Alteromonas sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
30% of maximum activity | Alteromonas sp. |
| 30 | 45 | - |
Alteromonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15 min, 30% residual activity | Alteromonas sp. |
| 50 | 90 | the agarase is heat-labile, with rapid loss of activity when treated at 50°C for 15 min or at 70°C for 1 min. It loses above 80% of its original activity after incubation at 90°C for 20 s | Alteromonas sp. |
| 70 | - |
1 min, 20% residual activity | Alteromonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Alteromonas sp. |
| 7 | - |
in presence of 2% NaCl | Alteromonas sp. |
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