Cloned (Comment) | Organism |
---|---|
gene Lp6-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time quantitative PCR expression analysis, cloning in Escherichia coli, recombinant expression in Pichia pastoris strain X33, secretion of the protein to the culture medium | Lolium perenne |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sucrose | 70-90% inhibition at 10-40 mM | Lolium perenne |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lolium perenne | D0PQE8 | gene Lp6-FEHa | - |
Lolium perenne Bravo | D0PQE8 | gene Lp6-FEHa | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme from Pichia pastoris strain X33 from the culture supernatant | Lolium perenne |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | following defoliation, during fructan breakdown, the enzyme transcript level decreases in elongating leaf bases and in mature leaf sheaths in parallel to increased total fructan exohydrolase activities | Lolium perenne | - |
root | - |
Lolium perenne | - |
stem | stubble | Lolium perenne | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,6-kestotetraose + H2O | - |
Lolium perenne | ? | - |
? | |
6,6-kestotetraose + H2O | - |
Lolium perenne Bravo | ? | - |
? | |
6-kestotriose + H2O | best substrate | Lolium perenne | ? | - |
? | |
6-kestotriose + H2O | best substrate | Lolium perenne Bravo | ? | - |
? | |
6G-kestotriose + H2O | lower activity | Lolium perenne | ? | - |
? | |
6G-kestotriose + H2O | lower activity | Lolium perenne Bravo | ? | - |
? | |
levan + H2O | lower activity | Lolium perenne | ? | - |
? | |
levan + H2O | lower activity | Lolium perenne Bravo | ? | - |
? | |
additional information | substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose | Lolium perenne | ? | - |
? | |
additional information | substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose | Lolium perenne Bravo | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-FEH | - |
Lolium perenne |
fructan 6-exohydrolase | - |
Lolium perenne |
Lp6-FEHa | - |
Lolium perenne |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to group I of glycosyl hydrolase famiyl 32, GH32, phylogenetic analysis, overview | Lolium perenne |
metabolism | regulation during development of fructan exohydrolases, overview | Lolium perenne |
physiological function | the enzyme is involved in the complex regulation of fructan breakdown in defoliated grassland species, which is a major forage grass species and accumulates fructans, mainly composed of beta(2,6)-linked fructose units. Fructans are mobilized through strongly increased activities of fructan exohydrolases | Lolium perenne |