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Literature summary for 3.2.1.80 extracted from

  • Lothier, J.; Van Laere, A.; Prudhomme, M.P.; Van den Ende, W.; Morvan-Bertrand, A.
    Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne (2014), Planta, 240, 629-643.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Lp6-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time quantitative PCR expression analysis, cloning in Escherichia coli, recombinant expression in Pichia pastoris strain X33, secretion of the protein to the culture medium Lolium perenne

Inhibitors

Inhibitors Comment Organism Structure
sucrose 70-90% inhibition at 10-40 mM Lolium perenne

Organism

Organism UniProt Comment Textmining
Lolium perenne D0PQE8 gene Lp6-FEHa
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Lolium perenne Bravo D0PQE8 gene Lp6-FEHa
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme from Pichia pastoris strain X33 from the culture supernatant Lolium perenne

Source Tissue

Source Tissue Comment Organism Textmining
leaf following defoliation, during fructan breakdown, the enzyme transcript level decreases in elongating leaf bases and in mature leaf sheaths in parallel to increased total fructan exohydrolase activities Lolium perenne
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root
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Lolium perenne
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stem stubble Lolium perenne
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,6-kestotetraose + H2O
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Lolium perenne ?
-
?
6,6-kestotetraose + H2O
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Lolium perenne Bravo ?
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?
6-kestotriose + H2O best substrate Lolium perenne ?
-
?
6-kestotriose + H2O best substrate Lolium perenne Bravo ?
-
?
6G-kestotriose + H2O lower activity Lolium perenne ?
-
?
6G-kestotriose + H2O lower activity Lolium perenne Bravo ?
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?
levan + H2O lower activity Lolium perenne ?
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?
levan + H2O lower activity Lolium perenne Bravo ?
-
?
additional information substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose Lolium perenne ?
-
?
additional information substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose Lolium perenne Bravo ?
-
?

Synonyms

Synonyms Comment Organism
6-FEH
-
Lolium perenne
fructan 6-exohydrolase
-
Lolium perenne
Lp6-FEHa
-
Lolium perenne

General Information

General Information Comment Organism
evolution the enzyme belongs to group I of glycosyl hydrolase famiyl 32, GH32, phylogenetic analysis, overview Lolium perenne
metabolism regulation during development of fructan exohydrolases, overview Lolium perenne
physiological function the enzyme is involved in the complex regulation of fructan breakdown in defoliated grassland species, which is a major forage grass species and accumulates fructans, mainly composed of beta(2,6)-linked fructose units. Fructans are mobilized through strongly increased activities of fructan exohydrolases Lolium perenne