Literature summary for 3.2.1.80 extracted from

Verhaest, M.; Lammens, W.; Le Roy, K.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.; Van den Ende, W.
Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor (2007), New Phytol., 174, 90-100.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Top10 cells	Cichorium intybus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour diffusion method, crystallization of complexes between 1-FEH IIa with the substrate 1-kestose and the inhibitors sucrose, fructose and 2,5-dideoxy-2,5-imino-D-mannitol	Cichorium intybus
mutant proteins are crystallized by hanging drop vapour diffusion method with 2.1 M sodium potassium phosphate and 0.1 M potassium phosphate, pH 7.0	Cichorium intybus

Protein Variants

Protein Variants	Comment	Organism
E201Q	inactive	Cichorium intybus
S101L	inactive	Cichorium intybus
S101L	no inhibition by sucrose	Cichorium intybus
W82L	inactive	Cichorium intybus
W82L	no inhibition by sucrose	Cichorium intybus

Inhibitors

Inhibitors	Comment	Organism	Structure
2,5 dideoxy-2,5-imino-D-mannitol	-	Cichorium intybus
D-fructose	-	Cichorium intybus
sucrose	63% inhibition by 10 mM sucrose, wild-type enzyme. Mutant enzymes W82L and S101L are not inhibited by sucrose. W82 and S101 are important for binding sucrose as inhibitor	Cichorium intybus

Organism

Organism	UniProt	Comment	Textmining
Cichorium intybus	Q93X60	precursor	-

Purification (Commentary)

Purification (Comment)	Organism
-	Cichorium intybus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-kestose + H2O	-	Cichorium intybus	?	-	?
1-kestose + H2O	-	Cichorium intybus	beta-D-fructose + sucrose	-	?

Synonyms

Synonyms	Comment	Organism
1-FEH IIa	-	Cichorium intybus
fructan 1-exohydrolase	-	Cichorium intybus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.5	-	sucrose	-	Cichorium intybus

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Release 2023.1 (January 2023)